文献类型： 外文期刊
作者： Liao, D. J. 1 ; Lu, X. P. 1 ; Chen, H. S. 1 ; Lu, Y. 2 ; Mo, Z. Y. 1 ;
作者机构： 1.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Dis, State Key Lab Resp Dis, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou, Peoples R China
关键词： Mango peel;Protein extraction;2-DE;Proteomic analysis;Evaluation
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2016 年 15 卷 3 期
页码：
收录情况： SCI
摘要： The peel of mango (Mangifera indica L.) is a special plant tissue that contains many compounds that interfere with protein extraction. A successful separation with Two-dimensional electrophoresis (2-DE) is the key step for proteomic analysis. To evaluate the efficiencies of mango peel protein extraction for 2-DE, four extraction methods were tested: 1) 2-D clean-up kit, 2) trichloroacetic acid/acetone precipitation, 3) phenol extraction, 4) phenol with methanol/ammonium acetate precipitation. The results showed that the phenol with methanol/ammonium acetate precipitation produced the best quality protein extraction and separation. Proteins were separated in 30-70 and > 70 kDa ranges better than with the other methods. Acidic proteins had better resolution with fewer horizontal and vertical streaks. Sixteen proteins were identified by maxtrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS/MS). The result demonstrated that each of these four methods can be used to prepare mango peel proteins. The phenol with methanol/ammonium acetate precipitation was the best choice for proteomic analysis of mango peel.
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