中国热带农业科学院机构知识库

Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

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文献类型：外文期刊

作者： Lu, Y. ¹ ; Xu, W. H. ³ ; Xie, Y. X. ² ; Zhang, X. ² ; Pu, J. J. ² ; Qi, Y. X. ² ; Li, H. P. ¹ ;

作者机构： 1.S China Agr Univ, Coll Nat Resources & Environm, Wushan, Peoples R China

2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Hainan, Peoples R China

3.Shangrao Normal Univ, Zhimin State In Shangrao, Jiangxi, Peoples R China

关键词： Resistance genes;NBS-LRR;Banana

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2011 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Commercial banana varieties are highly susceptible to fungal pathogens, as well as bacterial pathogens, nematodes, viruses, and insect pests. The largest known family of plant resistance genes encodes proteins with nucleotide-binding site (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for the isolation of candidate genes in banana that may be involved in plant defense. Six degenerate PCR primers were designed to target NBS and additional domains were tested on commercial banana species Musa acuminata subsp malaccensis and the Musa AAB Group propagated in vitro and plants maintained in a greenhouse. Total DNA was isolated by a modified CTAB extraction technique. Four resistance gene analogs were amplified and deposited in GenBank and assigned numbers HQ199833-HQ199836. The predicted amino acid sequences compared to the amino acid sequences of known resistance genes (MRGL1, MRGL2, MRGL3, and MRGL4) revealed significant sequence similarity. The presence of consensus domains, namely kinase-1a, kinase-2 and hydrophobic domain, provided evidence that the cloned sequences belong to the typical non-Toll/interleukin-1 receptor-like domain NBS-LRR gene family.

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