Determination of Aflatoxin B1 in Camellia Oleifera Seed by Immunoaffinity Column and High Performance Liquid Chromatography
文献类型： 外文期刊
作者： Lin, Ling 1 ; Yang, Chun Liang 1 ; Cha, Yu Bing 1 ; Bin Guo, Hong 1 ; Zeng, Ri Zhong 3 ;
作者机构： 1.CATAS, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China
2.Minist Agr Zhanjiang, Lab Qual Safety Risk Assessment Agr Prod Proc, Zhanjiang 524001, Peoples R China
3.CATAS, Rubber Res Inst, Danzhou 571737, Peoples R China
关键词： immunoaffinity column;HPLC;aflatoxin B1;camellia oleifera seed
期刊名称：FRONTIERS OF MANUFACTURING SCIENCE AND MEASURING TECHNOLOGY V
ISSN：
年卷期： 2015 年
页码：
收录情况： SCI
摘要： A method for the determination of aflatoxin B1 in camellia oleifera seed was established by immunoaffinity column and High Performance Liquid Chromatography. After being extracted with methanol-water, the sample extract was filtered, diluted with water and through to immunoaffinity column for cleaning, methanol dissolved the residues and then determination by post column derivatization HPLC fluorescence detection. Research results show that the detection limits of aflatoxin B1 reached 0.3 mu g/kg. The recovery were more than 85 %. And the sensitivity, accuracy and precision of this method were able to meet the requirements for sample analysis.
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