文献类型： 外文期刊

作者： Wu, F. H. ¹ ; Yu, X. D. ¹ ; Zhuang, N. S. ¹ ; Liu, G. D. ² ; Liu, J. P. ¹ ;

作者机构： 1.Hainan Univ, Coll Agron, Haikou, Hainan Province, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Crops Genet Resources, Danzhou, Hainan Province, Peoples R China

关键词： Stylosanthes guianensis;Tetraploid induction;Colchicine;Polyploid identification

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Stylosanthes guianensis is an elite and important forage legume species, which is extensively cultivated in tropical areas. Polyploid breeding via exposure to colchicine is a conventional and practical method to improve varieties of S. guianensis. Terminal buds of S. guianensis Reyan No. 5 seedlings were treated with different concentrations of colchicine (0.00, 0.05, 0.10, 0.15, 0.20, and 0.25%) for 24, 48, and 72 h. Morphological and cytological variants were observed at a frequency of <96% among transplanted seedlings. The cytogenetic analysis of young leaf cells was conducted on all variants to identify their ploidy levels. The most efficient procedure for tetraploid production was the treatment of seedling apical buds with 20% colchicine for 48 h, with the tetraploid induction rate being 10%. This is a relatively simple and reliable method for the production of tetraploidy in S. guianensis.