文献类型： 外文期刊
作者： Huang, C. Q. 1 ; Liu, G. D. 1 ; Bai, C. J. 1 ; Wang, W. Q. 1 ; Tang, J. 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Trop Crops Genet Resources Inst, Minist Agr, Danzhou 571737, Hainan, Peoples R China
关键词： Stylosanthes guianensis;Hybrid identification;SRAP markers;Male parent-specific markers
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Sequence-related amplified polymorphism (SRAP) is a new molecular marker technology developed based on polymerase chain reaction. The authenticity of 84 progenies of 8 hybrid combinations of Stylosanthes guianensis was identified by SRAP markers to select the true hybrids used in the present study. A total of 35 SRAP primer combinations were selected from the parents of 8 hybrid combinations. The selected polymorphism primer combinations were applied to identify the authenticity of all progenies. The male parents of the primer combinations had specific markers, whereas the female parents did not. 68 progenies exhibited male parent-specific bands, which were identified as true hybrids. The rest of the progenies were considered self-hybrids because of the absence of male parent-specific bands. The results of hybrid identification provided solid evidence for further studies of hybrids and demonstrated SRAP molecular markers as a useful technology for assessing the purity of S. guianensis hybrids.
- 相关文献
作者其他论文 更多>>
-
First Report of Cassava common mosaic virus Infecting Cassava in Mainland China
作者：Tuo, D. C.;Zhao, G. Y.;Yan, P.;Li, R. M.;Chen, X.;Wang, W. Q.;Li, X. Y.;Yao, Y.;Shen, W. T.;Zhou, P.;Tuo, D. C.;Yan, P.;Li, X. Y.;Shen, W. T.;Zhou, P.;Zhao, G. Y.;Cui, H. G.
关键词：cassava; Cassava common mosaic virus; China; complete genome
-
Establishment of a core collection of Cynodon based on morphological data
作者：Huang, C. Q.;Long, T.;Bai, C. J.;Wang, W. Q.;Tang, J.;Liu, G. D.
关键词：Germplasm conservation; optimal statistical strategy; phenotypic selection
-
Induction and identification of Stylosanthes guianensis tetraploids
作者：Wu, F. H.;Yu, X. D.;Zhuang, N. S.;Liu, J. P.;Liu, G. D.
关键词：Stylosanthes guianensis;Tetraploid induction;Colchicine;Polyploid identification
-
Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species
作者：Li, H. C.;Lu, H. B.;Yang, F. Y.;Liu, S. J.;Zhang, Y. W.;Li, H. C.;Lu, H. B.;Yang, F. Y.;Liu, S. J.;Zhang, Y. W.;Bai, C. J.
关键词：Pennisetum Rich.;Sucrose phosphate synthase;Gene clone;Sequence analysis
-
Genetic diversity of the Chinese traditional herb Blumea balsamifera (Asteraceae) based on AFLP markers
作者：Pang, Y. X.;Zhang, Y. B.;Yu, J. B.;Zhu, M.;Chen, Y. Y.;Wang, W. Q.;Yuan, Y.
关键词：Blumea balsamifera;Genetic diversity;AFLP;Conservation genetics
-
Grassland development in tropical and subtropical southern China
作者：Michalk, D. L.;Yu, D. G.;Liu, G. D.;Bai, C. J.;Chen, Z. Q.
关键词：biodiversity;productivity;rangelands;Stylosanthes
-
Genetic variation of asexual reproduction characteristics of Axonopus compressus (Sw.) P. Beauv.
作者：Wang, X. L.;Mo, D. Q.;Li, L. F.;Liao, L.;Wang, Z. Y.;Hu, H. G.;Bai, C. J.
关键词：
