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Isolation and characterization of microsatellite markers for Axonopus compressus (Sw.) Beauv. (Poaceae) using 454 sequencing technology

文献类型： 外文期刊

作者： Wang, X. -L. 1 ; Li, Y. 2 ; Liao, L. 1 ; Bai, C. -J. 3 ; Wang, Z. -Y. 1 ;

作者机构： 1.Hainan Univ, Key Lab Protect & Dev Utilizat Trop Crop Germplas, Minist Educ, Coll Agron, Haikou, Peoples R China

2.Henan Agr Univ, Coll Forestry, Zhengzhou, Peoples R China

3.Trop Pasture Res Ctr CATAS, Danzhou, Peoples R China

关键词： Microsatellite markers;Genetic diversity;Roche 454 sequencing;Axonopus compressus

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Axonopus compressus (Sw.) Beauv. is a perennial herb widely used as a garden lawn grass. In this study, we used Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO, to isolate simple sequence repeat markers from the A. compressus genome. A total of 1942 microsatellite loci were identified, with 53,193 raw sequencing reads. One hundred microsatellite loci were selected to test the primer amplification efficiency in 24 individuals; 14 primer pairs yielded polymorphic amplification products. The number of observed alleles ranged from two to six, with an average of 3.5. Shannon's Information index values ranged from 0.169 to 0.650, with an average of 0.393. Nei's genetic diversity values ranged from 0.108 to 0.457, with an average of 0.271. This first set of microsatellite markers developed for Axonopus will assist in the development of molecular marker-assisted breeding and the assessment of genetic diversity in A. compressus.

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