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Dual Functions in Response to Heat Stress and Spermatogenesis: Characterization of Expression Profile of Small Heat Shock Proteins 9 and 10 in Goat Testis

文献类型： 外文期刊

作者： Xun, Wenjuan 1 ; Shi, Liguang 1 ; Cao, Ting 1 ; Zhao, Chunping 1 ; Yu, Ping 1 ; Wang, Dingfa 1 ; Hou, Guanyu 1 ; Zhou, Ha 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci CATAS, Trop Crop Genet Resource Res Inst, Danzhou 571737, Hainan, Peoples R China

期刊名称：Biomed Research International （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2015 年

页码：

收录情况： SCI

摘要： Small heat shock proteins 9 and 10 (HSPB9 and HSPB10) are two testis-specific expressed sHsps. The objective of this study was to investigate the mRNA expression profile of HSPB9 and HSPB10 in goat testis among the different seasons, ages, and environmental temperatures. Allocation of the two sHsps was also performed by immunohistochemistry. The results showed that the transcript levels of HSPB9 and HSPB10 were extremely high in the testis (P < 0.01). The relative expression of HSBP9 and HSPB10 in testis showed a tendency to increase with age and then ismaintained at the constant level after sexualmaturity. HSPB9 and HSPB10 have significantly higher expression in the breeding season (P < 0.05) and hot season (P < 0.01). Both HSPB9 and HSPB10 were found to be upregulated by high-temperature stress in testis (P < 0.05), and the expressions of Hsp70 and Hsp90 were also increased simultaneously (P < 0.01). Immunohistochemistry analysis localized HSPB9 expressed in spermatogonia, spermatocytes, and round spermatids and HSPB10 expressed in the elongate spermatids. In epididymis, strongly staining signal ofHSPB10was detected in pseudostratified columnar epithelium. In conclusion, the two testis-specific sHsps are closely related to male reproduction and heat tolerance. The results could provide valuable data for the further studies on HSPB9 and HSPB10.

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