您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Large proportion of genes in one cryptic WO prophage genome are actively and sex-specifically transcribed in a fig wasp species

文献类型： 外文期刊

作者： Wang, Guan-Hong 1 ; Niu, Li-Ming 3 ; Ma, Guang-Chang 3 ; Xiao, Jin-Hua 1 ; Huang, Da-Wei 1 ;

作者机构： 1.Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou, Hainan, Peoples R China

4.Shandong Agr Univ, Plant Protect Coll, Tai An 271018, Shandong, Peoples R China

关键词： Defective prophage;Bacteriophage WO;Reverse-transcription PCR;Real-time quantitative PCR;Ankyrin-domain-containing protein

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2014 年 15 卷

页码：

收录情况： SCI

摘要： Background: Cryptic prophages are genetically defective in their induction and propagation, and are simply regarded as genetic remnants. There are several putative cryptic WO prophages in the sequenced Wolbachia genomes. Whether they are lytic is unclear and their functions are poorly understood. Only three open reading frames (ORFs) in cryptic WO prophages have been reported to be actively transcribed. Results: In this study, we comprehensively examined the transcription of the only cryptic WO prophage (WOSol) in a Wolbachia strain that infects a fig wasp, Ceratosolen solmsi (Agaonidae, Chalcidoidea). By analyzing the transcriptions of all the ORFs of WOSol in both sexes of C. solmsi, using qualitative and quantitative methods, we demonstrated that i) a high percentage of ORFs are actively transcribed (59%, 17/29); ii) the expression of these ORFs is highly sex-specific, with a strong male bias (three in females and 15 in males); iii) an ank (ankyrin-domain-containing) gene actively transcribed in both wasp sexes is more highly expressed in males. Conclusions: A large proportion of the genes in the cryptic WO prophage WOSol are expressed, which overturns the concept that cryptic prophages are simply genetically defective. The highly sex-specific expression patterns of these genes in the host suggest that they play important roles in Wolbachia biology and its reproductive manipulation of its insect host, particularly through the males.

  • 相关文献

[1]Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea). Zhou, Ying,Li, Jing,Cheng, Jun,Zhou, Hui,Gu, Chao,Han, Yue-Peng,Guo, Dong,Gardiner, Sue. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台