您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Kocuria rosea HN01, a newly alkaliphilic humus-reducing bacterium isolated from cassava dreg compost

文献类型： 外文期刊

作者： Wu, Chun-Yuan 1 ; Chen, Nan 1 ; Li, Hong 1 ; Li, Qin-Fen 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Peoples R China

2.Hainan Univ, Coll Environm & Plant Protect, Haikou 570228, Peoples R China

3.Danzhou Sci Observing & Expt Stn Agroenvironm, Minist Agr, Danzhou 571737, Peoples R China

关键词： Dechlorination;Humus-reducing bacterium;Iron(III) oxides;Kocuria rosea;Organochlorine pollutants;Reductive transformation

期刊名称：JOURNAL OF SOILS AND SEDIMENTS （ 影响因子：3.308； 五年影响因子：3.586 ）

ISSN： 1439-0108

年卷期： 2014 年 14 卷 2 期

页码：

收录情况： SCI

摘要： The aims of this study were to isolate an alkaliphilic humus-reducing bacterium, investigate the fastest microbial reduction of humus analog as affected by different cultivation, and examine its ability for iron(III) oxide reduction and organochlorine pollutants (OCPs) degradation. A strain of pure culture, designated as HN01, was isolated from cassava dreg compost using anaerobic enrichment procedure with glucose as the electron donor and anthraquinone-2,6-disulphonate (AQDS) as the sole terminal electron acceptor. The isolate strain was identified using phenotypic and phylogenetic analysis. Iron(III) oxides and OCPs were chosen as potential electron acceptors. Strict anaerobic techniques and sterile conditions were applied throughout the incubation experiments, purged with O-2-free N-2 for 15 min. The concentration of reduced AQDS and Fe(II) was then quantified using a UV-vis spectrophotometer. The concentration of OCPs was analyzed by gas chromatography with a micro-electron capture detector. Cell number was determined by direct plate counting on aerobic Luria-Bertani medium agar medium at pH 9. (1) Strain HN01 was identified as Kocuria rosea, and the AQDS reduction by HN01 was observed in NaCl concentrations below 12 % (w/v) (optimum, 10 %) and pH ranges of 6.0-10.0 (optimum, 9.0) with sucrose as electron donor at 30 A degrees C; (2) glucose, sucrose, methanol, ethanol, glycerol, and acetate were the favorable electron donors for AQDS reduction by strain HN01; (3) the strain had the ability of reducing iron(III) oxides in the presence of sucrose at pH 9.0 and its Fe(III)-reducing capacity ranked as goethite (alpha-FeOOH) > lepidocrocite (gamma-FeOOH) > haematite(alpha-Fe2O3); and (4) the strain could effectively dechlorinate p,p'-DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane), and the dechlorination rate reached 71.3 %. This is the first report of a strain of K. rosea capable of reducing AQDS, iron (III) oxides, and p,p'-DDT, which extends the diversity of the alkaliphilic and halotolerant humus/Fe(III)-reducing bacterium associated with dechlorination. The strain may have the potential to be used for bioremediation of an anoxic alkaline wastewater or site contaminated with OCPs.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台