文献类型： 外文期刊

作者： Luo, C. ¹ ; Zhang, Q. L. ¹ ; Luo, Z. R. ¹ ;

作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan, Peoples R China

2.Chinese Acad Trop Agr Sci, Minist Agr, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Zhanjiang, Peoples R China

关键词： Diospyros kaki Thunb.;Simple sequence repeats;Roche 454 sequencing

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 2 期

页码：

收录情况： SCI

摘要： Oriental persimmon (Diospyros kaki Thunb.) (2n = 6x = 90) is a major commercial and deciduous fruit tree that is believed to have originated in China. However, rare transcriptomic and genomic information on persimmon is available. Using Roche 454 sequencing technology, the transcriptome from RNA of the flowers of D. kaki was analyzed. A total of 1,250,893 reads were generated and 83,898 unigenes were assembled. A total of 42,711 SSR loci were identified from 23,494 unigenes and 289 polymerase chain reaction primer pairs were designed. Of these 289 primers, 155 (53.6%) showed robust PCR amplification and 98 revealed polymorphism between 15 persimmon genotypes, indicating a polymorphic rate of 63.23% of the productive primers for characterization and genotyping of the genus Diospyros. Transcriptome sequence data generated from next-generation sequencing technology to identify microsatellite loci appears to be rapid and cost-efficient, particularly for species with no genomic sequence information available.