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Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango

文献类型: 外文期刊

作者: Luo, C. 1 ; Wu, H. X. 1 ; Yao, Q. S. 1 ; Wang, S. B. 1 ; Xu, W. T. 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang, Peoples R China

关键词: Mango;EST-SSR;TRAP;Genetic analysis

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 3 期

页码:

收录情况: SCI

摘要: Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7 mango genotypes with amplicons ranging from 84 to 160 bp; 93 of the primer pairs yielded polymorphic products. The proportion of polymorphic bands ranged from 16.67 to 100%, with a mean of 55.64%. In contrast, 86 primer pairs exhibited good amplification with clear bands for target region amplification polymorphism analysis, and a total of 66 primer combinations were polymorphic. These two novel sets of EST-derived markers will be of use in future studies of genetic diversity, genetic map construction, and marker-assisted selection in mango.

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