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Comparative proteomics analysis revealed increased expression of photosynthetic proteins in transgenic tobacco by overexpression of AtCBF1 gene

文献类型: 外文期刊

作者: Cheng, Han 1 ; Cai, Haibin 1 ; An, Zewei 1 ; Huang, Huasun 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou City 571737, Hainan, Peoples R China

关键词: CRT/DRE binding factor 1 (CBF1);cold stress;proteomics;photosynthesis;tobacco

期刊名称:PLANT OMICS ( 影响因子:0.777; 五年影响因子:0.802 )

ISSN: 1836-0661

年卷期: 2013 年 6 卷 3 期

页码:

收录情况: SCI

摘要: CRT/DRE Binding Factor 1 (CBF1) overexpression enhanced freezing tolerance in Nicotiana tabacum. To unravel the proteomics changes caused by the ectopic overexpression of AtCBF1 genes, we utilized 2-DE along with MALDI-TOF-MS and LC ESI-MS/MS to study the proteome profiles. We also tried to identify the proteins that significantly change in abundance in the AtCBF1 transgenic tobacco compared to wild type plant. Totally, 68 proteins were up-regulated in AtCBF1 transgenic tobacco, while 150 were downregulated when the plants were cultured at room temperature. Sixteen up-regulated proteins were successfully identified and functionally annotated. These proteins mainly fell into 4 categories, including photosynthetic proteins, metabolic proteins, stress related proteins and chaperons. In all identified proteins, the photosynthetic proteins were the major category, suggesting AtCBF1 overexpression has substantial positive effects on photosynthesis system. The chlorophyll a and b content were substantially increased by 20% and 40% in transgenic tobacco than in wild type plant, respectively. These data suggested that AtCBF1 ectopic overexpression protected tobacco photosynthetic system under cold stress, which contributes the cold tolerance of this non-cold acclimation plant.

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