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Optimization of Ultrasonic Extraction of Total Flavonoids from Litchi (Litchi chinensis Sonn.) Seed by Response Surface Methodology

文献类型： 外文期刊

作者： Lin, Lijing 1 ; Huang, Xiaobing 1 ; Li, Jihua 1 ; Zhou, Yaomin 1 ; Tang, Yongfu 1 ; Ran, Xu 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China

关键词： Response surface methodology (RSM);litchi seed;total flavonoids;ultrasonic extraction

期刊名称：ADVANCES IN CHEMICAL, MATERIAL AND METALLURGICAL ENGINEERING, PTS 1-5

ISSN： 1022-6680

年卷期： 2013 年 634-638 卷

页码：

收录情况： SCI

摘要： The ultrasonic technology was applied to optimize the processing parameters of extraction of flavonoids from litchi seed using the response surface methodology. The Box-Behnken design was used to analyze the effect of independent variables including extraction temperature (Xi), methanol concentration (X-2) and extraction time (X-3) on the yield of total flavonoids as the dependent variable(Y). The analysis of variance (ANOVA) indicated that three independent variables, their quadratic terms and interaction terms showed a significant effect on yield of total flavonoids (YTF) except the interaction between X-1 and X-2. A mathematical model with high determination coefficient was constructed, according to which the optimal extraction conditions were determined as follows: extraction temperature 70 degrees C, methanol concentration 68%, extraction time 70 min. Under the above-mentioned condition, the experimental value of YTF was 3.55 g catechin equivalent per 100 g of dry (g CE/100 g), which agreed with the predicted value perfectly, demonstrating the response surface model was suitable in optimizing the extraction of flavonoids from litchi seed.

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