Identifying pathogenicity genes in the rubber tree anthracnose fungus Colletotrichum gloeosporioides through random insertional mutagenesis
文献类型: 外文期刊
作者: Cai, Zhiying 1 ; Li, Guohua 3 ; Lin, Chunhua 2 ; Shi, Tao 2 ; Zhai, Ligang 4 ; Chen, Yipeng 2 ; Huang, Guixiu 2 ;
作者机构: 1.Hainan Univ, Environm & Plant Protect Coll, Danzhou 571737, Hainan, Peoples R China
2.CATAS, Environm & Plant Protect Inst, Danzhou 571737, Hainan, Peoples R China
3.Yunnan Inst Trop Crops, Jinghong 666100, Yunnan, Peoples R China
4.Huazhong Agr Univ, Plant Sci & Technol Coll, Wuhan 430070, Hubei, Peoples R China
关键词: Fungal transformation;Insertional mutagenesis;Pathogenicity gen;Anthracnose;Colletotrichum gloeosporioides
期刊名称:MICROBIOLOGICAL RESEARCH ( 影响因子:5.415; 五年影响因子:6.038 )
ISSN: 0944-5013
年卷期: 2013 年 168 卷 6 期
页码:
收录情况: SCI
摘要: To gain more insight into the molecular mechanisms of Colletotrichum gloeosporioides pathogenesis, Agrobacterium tumefaciens-mediated transformation (ATMT) was used to identify mutants of C. gloeosporioides impaired in pathogenicity. An ATMT library of 4128 C. gloeosporioides transformants was generated. Transformants were screened for defects in pathogenicity with a detached copper brown leaf assay. 32 mutants showing reproducible pathogenicity defects were obtained. Southern blot analysis showed 60.4% of the transformants had single-site T-DNA integrations. 16 Genomic sequences flanking 1-DNA were recovered from mutants by thermal asymmetric interlaced PCR, and were used to isolate the tagged genes from the genome sequence of wild-type C gloeosporioides by Basic Local Alignment Search Tool searches against the local genome database of the wild-type C. gloeosporioides. One potential pathogenicity genes encoded calcium-translocating P-type ATPase. Six potential pathogenicity genes had no known homologs in filamentous fungi and were likely to be novel fungal virulence factors. Two putative genes encoded Glycosyltransferase family 28 domain-containing protein and Mov34/MPN/PAD-1 family protein, respectively. Five potential pathogenicity genes had putative function matched with putative protein of other Colletotrichum species. Two known C. gloeosporioides pathogenicity genes were also identified, the encoding GlomerelIa cingulata hard-surface induced protein and C gloeosporioides regulatory subunit of protein kinase A gene involved in cAMP-dependent PKA signal transduction pathway. (C) 2013 Elsevier GmbH. All rights reserved.
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