The interaction of banana MADS-box protein MuMADS1 and ubiquitin-activating enzyme E-MuUBA in post-harvest banana fruit
文献类型: 外文期刊
作者: Liu, Ju-Hua 1 ; Zhang, Jing 1 ; Jia, Cai-Hong 1 ; Zhang, Jian-Bin 1 ; Wang, Jia-Shui 2 ; Yang, Zi-Xian 1 ; Xu, Bi-Yu 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Key Lab Trop Crop Biotechnol, Minist Agr, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China
2.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou 570102, Peoples R China
3.Chinese Acad Trop Agr Sc
关键词: Banana (Musa acuminata L. AAA group, cv. Brazilian);Yeast two-hybrid;MuMADS1;MuUBA;Interaction;Post-harvest ripening
期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )
ISSN: 0721-7714
年卷期: 2013 年 32 卷 1 期
页码:
收录情况: SCI
摘要: The interaction of MuMADS1 and MuUBA in banana was reported, which will help us to understand the mechanism of the MADS-box gene in regulating banana fruit development and ripening. The ubiquitin-activating enzyme E1 gene fragment MuUBA was obtained from banana (Musa acuminata L.AAA) fruit by the yeast two-hybrid method using the banana MADS-box gene MuMADS1 as bait and 2-day post-harvest banana fruit cDNA library as prey. MuMADS1 interacted with MuUBA. The interaction of MuMADS1 and MuUBA in vivo was further proved by bimolecular fluorescence complementation assay. Real-time quantitative PCR evaluation of MuMADS1 and MuUBA expression patterns in banana showed that they are highly expressed in the ovule 4 stage, but present in low levels in the stem, which suggests a simultaneously differential expression action exists for both MuMADS1 and MuUBA in different tissues and developmental fruits. MuMADS1 and MuUBA expression was highly stimulated by exogenous ethylene and suppressed by 1-methylcyclopropene. These results indicated that MuMADS1 and MuUBA were co-regulated by ethylene and might play an important role in post-harvest banana fruit ripening.
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