Diversity and chemotaxis of soil bacteria with antifungal activity against Fusarium wilt of banana
文献类型: 外文期刊
作者: Li, Ping 1 ; Ma, Li 1 ; Feng, Yun Li 1 ; Mo, Ming He 1 ; Yang, Fa Xiang 3 ; Dai, Hao Fu 4 ; Zhao, You Xing 4 ;
作者机构: 1.Yunnan Univ, Lab Conservat & Utilizat Bioresources, Minist Educ, Kunming 650091, Peoples R China
2.Yunnan Univ, Key Lab Microbial Resources, Minist Educ, Kunming 650091, Peoples R China
3.Yunnan Microbial Fermentat Engn Res Ctr Co Ltd, Kunming 650217, Peoples R China
4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China
关键词: Fusarium oxysporum f. sp cubense;Banana;Biological control bacteria;Chemotaxis;Root exudates
期刊名称:JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.346; 五年影响因子:3.426 )
ISSN: 1367-5435
年卷期: 2012 年 39 卷 10 期
页码:
收录情况: SCI
摘要: The chemotactic response of bacteria to root exudates plays an important role in the colonization of bacteria in the rhizosphere. In this study, 420 strains of antifungal bacteria against Fusarium oxysporum f. sp. cubense (Foc) were screened for chemotaxis based on a cheA molecular diagnostic method. A total of 124 strains with antifungal efficiencies of 27.26-67.14 % generated a characteristic band of cheA. The chemotaxis of 97 bacterial strains producing a cheA band was confirmed using the drop assay and swarm plate assay using catechol, p-hydroxybenzoic acid, salicylic acid, and asparagine as the attractants. A phylogenetic analysis based on restriction fragment length polymorphisms (RFLPs) and 16S rDNA sequences indicated that the 124 chemotactic antagonists of Foc were affiliated with 18 species of Paenibacillaceae, Bacillaceae, Streptomycineae, Enterobacteriaceae, and Pseudomonadaceae. The chemical composition of banana root exudates were analyzed by GC-MS, and 62 compounds, including alkanes, alkenes, naphthalenes, benzenes, and alcohols, were evaluated. Five representative antagonists of Foc showed 1.76- to 7.75-fold higher chemotactic responses than the control to seven compounds in banana root exudates, as determination by capillary assays.
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