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Expression Patterns, Activities and Carbohydrate-Metabolizing Regulation of Sucrose Phosphate Synthase, Sucrose Synthase and Neutral Invertase in Pineapple Fruit during Development and Ripening

文献类型: 外文期刊

作者: Zhang, Xiu-Mei 1 ; Wang, Wei 3 ; Du, Li-Qing 1 ; Xie, Jiang-Hui 1 ; Yao, Yan-Li 1 ; Sun, Guang-Ming 1 ;

作者机构: 1.CATAS, Key Lab Trop Fruit Biol, Minist Agr, S Subtrop Crop Res Inst, Zhanjiang 524091, Guangdong, Peoples R China

2.Lousiana State Univ, Dept Food Sci, Baton Rouge, LA 70803 USA

3.Anhui Agr Univ, Sch Life Sci, Lab Plant Genet & Breeding, Hefei 230036, Anhui, Peoples R China

关键词: Ananas comosusr;carbohydrate metabolism;enzyme activties;gene cloning;transcript expression

期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )

ISSN: 1661-6596

年卷期: 2012 年 13 卷 8 期

页码:

收录情况: SCI

摘要: Differences in carbohydrate contents and metabolizing-enzyme activities were monitored in apical, medial, basal and core sections of pineapple (Ananas comosus cv. Comte de paris) during fruit development and ripening. Fructose and glucose of various sections in nearly equal amounts were the predominant sugars in the fruitlets, and had obvious differences until the fruit matured. The large rise of sucrose/hexose was accompanied by dramatic changes in sucrose phosphate synthase (SPS) and sucrose synthase (SuSy) activities. By contrast, neutral invertase (NI) activity may provide a mechanism to increase fruit sink strength by increasing hexose concentrations. Furthermore, two cDNAs of Ac-sps (accession no. GQ996582) and Ac-ni (accession no. GQ996581) were first isolated from pineapple fruits utilizing conserved amino-acid sequences. Homology alignment reveals that the amino acid sequences contain some conserved function domains. Transcription expression analysis of Ac-sps, Ac-susy and Ac-ni also indicated distinct patterns related to sugar accumulation and composition of pineapple fruits. It suggests that differential expressions of multiple gene families are necessary for sugar metabolism in various parts and developmental stages of pineapple fruit. A cycle of sucrose breakdown in the cytosol of sink tissues could be mediated through both Ac-SuSy and Ac-NI, and Ac-NI could be involved in regulating crucial steps by generating sugar signals to the cells in a temporally and spatially restricted fashion.

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