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Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross-linking

文献类型： 外文期刊

作者： Liu Yuge 1 ; Li Weiming 1 ; Wei Changbin 1 ; Lu Lingling 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, S Subtrop Crops Res Inst, Zhanjiang 524091, Guangdong, Peoples R China

关键词： sensor;xanthine oxidase;chitosan;cross-linking

期刊名称：CHINESE JOURNAL OF CHEMISTRY （ 影响因子：6.0； 五年影响因子：3.19 ）

ISSN： 1001-604X

年卷期： 2012 年 30 卷 7 期

页码：

收录情况： SCI

摘要： Here in this paper, xanthine oxidase (XOD) was immobilized onto the chitosan (CHT) modified electrode by a simple way of cross-linking with glutaraldehyde (GTD) and 3-aminopropyltriethoxysilane (KH). The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 mu mol/L with a low detection limit of 0.0215 mu mol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxanthine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.

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