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Cloning and expression analysis of a partial LEAFY homologue from pineapple (Ananas comosus (L.) Merr.)

文献类型： 外文期刊

作者： Lv Lingling 1 ; Sun Guangming 1 ; Xie Jianghui 2 ; Zhang Jianxia 1 ; Liu Shenghui 2 ; Liu Yuge 2 ; Wei Changbin 2 ; Zeng 1 ;

作者机构： 1.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, S Subtrop Crops Res Inst, Zhanjiang 524091, Guangdong, Peoples R China

3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

4.Natl Ctr Important Trop Crops Engn & Technol Re

关键词： Pineapple;cloning;expression;RT-PCR;LEAFY

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 71 期

页码：

收录情况： SCI

摘要： LEAFY-like genes are crucial regulators of flowering in angiosperms. A partial homologue of LEAFY, designated as AcLFY (Genebank accession no HQ433335), was isolated from pineapple (Ananas comosus L. cv. Comte de Paris) by PCR. The conserved cDNA fragment of AcLFY is 256 bp in length and contained an open reading frame of 248 bp, which encodes 82 amino acids protein. The amino acid alignment showed that AcLFY had a high identity with the related fragment of PhalLFY in Phalaenopsis hybrid cultivar (91%), LFY in Oncidium (90%) and OrcLFY in Orchis italica (88%). RT-PCR analysis showed that the AcLFY could be expressed constitutionally. Moreover, it was expressed in the flesh of young fruit and bract at low levels, and was highly expressed in the fruit, stem, petal and sepal.

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