A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin in the nanosheets of exfoliated HNb3O8
文献类型： 外文期刊
作者： Liu, Yu-Ge 1 ; Wei, Chang-bin 1 ; Lv, Ling-Ling 1 ; Liu, Sheng-Hui 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, S Subtrop Crops Res Inst, Zhanjiang 524091, Peoples R China
关键词： Hemoglogin;Direct electron transfer;Layered HNb3O8;Hydrogen peroxide;Biosensor
期刊名称：JOURNAL OF SOLID STATE ELECTROCHEMISTRY （ 影响因子：2.647； 五年影响因子：2.53 ）
ISSN： 1432-8488
年卷期： 2012 年 16 卷 6 期
页码：
收录情况： SCI
摘要： In the present work, hemoglobin (Hb) was entrapped into the nanosheets of a pre-exfoliated layered material HNb3O8. UV-vis spectra analysis displayed that no significant denaturation occurred to the entrapped protein. Electrochemical results showed that the entrapment of Hb into layered HNb3O8 enhanced the direct electron transfer ability between protein molecules and electrode. A pair of well-defined redox peaks was observed at -0.39 and -0.34 V on the glassy carbon electrode modified with the Hb/HNb3O8 composite. The electrode reactions showed a surface-controlled process with a single electron transfer at the scan rate of 50-400 mV/s, and the electron transfer rate was very fast. The entrapped Hb retained its biological activity well and the sensor constructed by the Hb/HNb3O8-composite-modified electrode displayed excellent response to the reduction of hydrogen peroxide (H2O2) with wide linear range, low detection limit, and good stability.
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