文献类型： 外文期刊

作者： Han, Bin ¹ ; Xu, Sheng ² ; Xie, Yan-Jie ² ; Huang, Jing-Jing ² ; Wang, Li-Juan ² ; Yang, Zheng ² ; Zhang, Chang-He ³ ; Su ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Hainan 571737, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

3.Univ Tras Os Montes & Alto Douro UTAD, Ctr Res & Technol Agroenvironm & Biol Sci CITAB, Dept Biol & Environm, P-5001801 Vila Real, Portugal

关键词： Cell cycle regulatory genes;Haem oxygenase-1;Lateral root formation;Maize (Zea mays L.);Transgenic Arabidopsis plants

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2012 年 184 卷

页码：

收录情况： SCI

摘要： Previous results revealed that haem oxygenase-1 (HO-1)/carbon monoxide (CO) system is involved in auxin-induced adventitious root formation. In this report, a cDNA for the gene ZmHO-1, encoding an HO-1 protein, was cloned from Zea mays seedlings. ZmHO-1 has a conserved HO signature sequence and shares highest homology with rice SE5 (OsHO-1) protein. We further discovered that N-1-naphthylacetic acid (NAA), haemin, and CO aqueous solution, led to the induction of ZmHO-1 expression as well as the thereafter promotion of lateral root development. These effects were specific for ZmHO-1 since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) differentially blocked the above actions. The addition of haemin and CO were able to reverse the auxin depletion-triggered inhibition of lateral root formation as well as the decreased ZmHO-1 transcripts. Molecular evidence showed that the haemin- or CO-mediated the modulation of target genes responsible for lateral root formation, including ZmCDK and ZmCK12, could be blocked by ZnPPIX. Overexpression of ZmHO-1 in transgenic Arabidopsis plants resulted in promotion of lateral root development as well as the modulation of cell cycle regulatory gene expressions. Overall, our results suggested that a maize HO-1 gene is required for the lateral root formation. (C) 2011 Elsevier Ireland Ltd. All rights reserved.