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Improve lipid production by pH shifted-strategy in batch culture of Chlorella protothecoides

文献类型： 外文期刊

作者： Liang, Guobin 2 ; Mo, Yiwei 1 ; Tang, Jianghong 2 ; Zhou, Quanfan 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, S Subtrop Crop Res Inst, Zhanjiang 524091, Peoples R China

2.Jiangsu Teachers Univ Technol, Sch Chem & Environm Engn, Changzhou 213001, Peoples R China

关键词： Heterotrophic cultivation;lipid;ph-shift strategy;glucose and Chlorella protothecoides

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2011 年 5 卷 28 期

页码：

收录情况： SCI

摘要： Effects of culture broth pH values on cells growth and lipid production were investigated in heterotrophic cultivation of Chlorella protothecoides. Based on the phenomenon that lower pH value favors cells growth but retards lipid synthesis and vice versa for higher pH value, a novel pH-shift strategy, optimized via simulating Gauss function, was developed. By controlling pH at 5.0 for first 93 h and switching to 6.5 afterwards, final lipid yield and productivity reached 3.75 g/L and 20.8 mg/L/h, increased by 24.2 and 38.7%, respectively as compared to constant pH 6.0 operations. Moreover, by feeding glucose and aqueous ammonia replacing sulphuric acid (H(2)SO4) and NaOH solutions to control pH, maximal lipid yield of 3.96 g/L was achieved, suggesting application of the novel pH-shift strategy for lipid overproduction as being feasible.

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