The Phytoalexin Resveratrol Regulates the Initiation of Hypersensitive Cell Death in Vitis Cell
文献类型: 外文期刊
作者: Chang, Xiaoli 1 ; Heene, Ernst 1 ; Qiao, Fei 2 ; Nick, Peter 1 ;
作者机构: 1.Karlsruhe Inst Technol, Bot Inst 1, Dept Mol Cell Biol, Karlsruhe, Germany
2.Chinese Acad Trop Agr Sci, Inst Trop Crops Genet Resources, Danzhou, Peoples R China
期刊名称:PLOS ONE ( 影响因子:3.24; 五年影响因子:3.788 )
ISSN: 1932-6203
年卷期: 2011 年 6 卷 10 期
页码:
收录情况: SCI
摘要: Resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance. The resistance of North American grapevine Vitis rupestris is correlated with a hypersensitive reaction (HR), while susceptible European Vitis vinifera cv. 'Pinot Noir' does not exhibit HR, but expresses basal defence. We have shown previously that in cell lines derived from the two Vitis species, the bacterial effector Harpin induced a rapid and sensitive accumulation of stilbene synthase (StSy) transcripts, followed by massive cell death in V. rupestris. In the present work, we analysed the function of the phytoalexin resveratrol, the product of StSy. We found that cv. 'Pinot Noir' accumulated low resveratrol and its glycoside trans-piceid, whereas V. rupestris produced massive trans-resveratrol and the toxic oxidative delta-viniferin, indicating that the preferred metabolitism of resveratrol plays role in Vitis resistance. Cellular responses to resveratrol included rapid alkalinisation, accumulation of pathogenesis-related protein 5 (PR5) transcripts, oxidative burst, actin bundling, and cell death. Microtubule disruption and induction of StSy were triggered by Harpin, but not by resveratrol. Whereas most responses proceeded with different amplitude for the two cell lines, the accumulation of resveratrol, and the competence for resveratrol-induced oxidative burst differed in quality. The data lead to a model, where resveratrol, in addition to its classical role as antimicrobial phytoalexin, represents an important regulator for initiation of HR-related cell death.
- 相关文献
作者其他论文 更多>>
-
Two types of coumarins-specific enzymes complete the last missing steps in pyran- and furanocoumarins biosynthesis
作者:Zhao, Yucheng;Zhang, Libo;Zhao, Yucheng;Zhang, Libo;Xiao, Yibei;He, Yuedong;Han, Liangliang;Xia, Yuanzheng;Yin, Fucheng;Wang, Xiaobing;Kong, Lingyi;Han, Liangliang;Xia, Yuanzheng;Yin, Fucheng;Wang, Xiaobing;Kong, Lingyi;Zhao, Deqing;Qiao, Fei;Xu, Sheng;Xiao, Yibei
关键词:Tetrahydropyrans; Tetrahydrofurans; Baldwin rules; Coumarins; CYP450 cyclase
-
Physiological and Transcriptomic Analyses Reveal the Mechanisms Underlying Methyl Jasmonate-Induced Mannitol Stress Resistance in Banana
作者:Yu, Jiaxuan;Tang, Lu;Li, Xinguo;Yu, Jiaxuan;Liu, Juhua;Li, Xinguo;Qiao, Fei
关键词:banana; MeJA; RNA-seq; mannitol-induced stress
-
The gradual establishment of complex coumarin biosynthetic pathway in Apiaceae
作者:Huang, Xin-Cheng;Wei, Xuefen;Hu, Shuaiya;Wu, Jia-Yi;Xue, Jia-Yu;Tang, Huanying;Zhao, Yucheng;Tang, Huanying;Zhao, Yucheng;He, Yuedong;Xu, Dingqiao;Qiao, Fei;Qiao, Fei;Zhao, Yucheng
关键词:
-
Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells
作者:Yan, Manwen;Ding, Zhuoying;Jiang, Xuefei;Yan, Manwen;Li, Mingyan;Ding, Zhuoying;Jiang, Xuefei;Qiao, Fei
关键词:Citrullus lanatus; osmotic stress; melatonin; plant hormone signals; gene expression
-
Forming coumarin C-glycosides via biocatalysis: Characterization of a C-glycosyltransferase from Angelica decursiva
作者:Wang, Zhiming;Jiang, Xuefei;Wang, Zhiming;Jiang, Xuefei;He, Yuedong;Liao, Lijing;Xiao, Yibei;Zhang, Yuhao;Zhao, Yucheng;Qiao, Fei
关键词:Angelica decursiva; C-glycosyltransferase; Coumarin; Catalytic mechanism; Molecular docking
-
Functional characterization of a Colchicum autumnale L. double-bond reductase (CaDBR1) in colchicine biosynthesis
作者:Xiong, Zhiqiang;Wang, Liang;Sun, Jingyi;Cong, Hanqing;Sun, Huapeng;Qiao, Fei;Xiong, Zhiqiang;Wang, Liang;Sun, Jingyi;Jiang, Xuefei
关键词:Colchicum autumnale L; Alkenal double-bond reductase; 4-HDCA; Functional characterization
-
Selection and Identification of a Reference Gene for Normalizing Real-Time PCR in Mangos under Various Stimuli in Different Tissues
作者:Yao, Rundong;Huang, Xiaolou;Cong, Hanqing;Qiao, Fei;Chen, Yeyuan;Yao, Rundong;Cheng, Yunjiang;Chen, Yeyuan
关键词:Mangifera indica L; real-time PCR; reference gene; gene expression; normalization