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Identification and characterization of differential gene expression in the mesocarp and kernel of oil palm nuts using suppression subtractive hybridization

文献类型： 外文期刊

作者： Xu, Li 2 ; Yuan, Yijun 1 ; Zhang, Lin 1 ; Wan, Li 1 ; Zheng, Yusheng 1 ; Zhou, Peng 4 ; Li, Dongdong 1 ;

作者机构： 1.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Hainan 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Hainan 571737, Danzhou, Peoples R China

3.Minist Agr, Key Lab Trop Crops Germplasm Resources Utilizat, Hainan 571737, Danzhou, Peoples R China

4.Chinese Acad Trop Agr Sci, Key Lab Trop Crop Biotechnol, Minist Agr, Inst Trop Biosci & Biotechnol, Hainan 571737, Danzhou, Peoples R China

5.Chinese Acad Trop Agr Sci, Anal & Testing Ctr, Hainan 571737, Danzhou, Peoples R China

关键词： Mesocarp;Kernel;Oil palm (Elaeis guineensis Jacq.);Real-time PCR;Suppression subtracted hybridization

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2011 年 7 卷 5 期

页码：

收录情况： SCI

摘要： Suppression subtracted hybridization (SSH) and dot blotting were used to identify differential gene expression in the mesocarp and kernel of oil palm nuts. The different types of nut tissue show differences in fatty acid anabolism and the synthesis of other important compounds. In total, 302 clones from forward SSH libraries and 238 clones from reverse SSH libraries were identified following differential screening, respectively. Among these, 120 clones from the forward SSH library and 81 clones from the reverse SSH library, showed tenfold or more differential expression levels, and were sequenced. Sequence analysis revealed that 76 clones (28 from the forward SSH library and 48 from the reverse SSH library) represent non-redundant cDNA inserts. The differential expression of 39 subset genes in the two different tissues was further confirmed by RT-PCR analysis. Functionally annotated blasting against the GenBank non-redundant protein database classified all 76 candidate genes into six categories, according to their putative functions. Interestingly, our results show that a group of significantly differentially expressed genes are involved in processes associated with oil palm nut maturation, such as the synthesis of medium-chain saturated fatty acids and phytic acid, nut development, and stress/defense responses. This study describes some relationships between gene expression and metabolic pathways in mature oil palm nuts, and contributes to our understanding of oil palm nut ESTs.

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