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Plant regeneration system from cotyledons-derived calluses cultures of Stylosanthes guianensis cv. 'Reyan 2'

文献类型： 外文期刊

作者： Yuan, Xuejun 1 ; Liao, Li 4 ; Liu, Guodao 1 ; Wang, Zhiyong 3 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Trop Crops Germplasm Resources Utilizat, Minist Agr, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China

2.Qiongzhou Univ, Coll Life Sci, Sanya 572000, Peoples R China

3.Hainan Univ, Minist Educ, Key Lab Protect & Dev Utilizat Trop Crop Germplas, Haikou 570228, Peoples R China

4.Hainan Univ, Coll Agr, Danzhou 571737, Peoples R China

关键词： Stylosanthes guianensis Sw. cv. Reyan 2;callus;cotyledons;plant regeneration

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 56 期

页码：

收录情况： SCI

摘要： The objective of this study was to successfully establish plant regeneration system with cotyledons of Stylosanthes guianensis Sw. cv. 'Reyan 2' as explants. In this study, the following results were obtained; (1) the highest rates of callus induction on medium MS with 3.0 mg L(-1) 2, 4-D with cotyledons as explants were 74%. The above medium of formulation was adopted for subculture; (2) the highest rates of callus differentiation medium MS with 3.0 mg L(-1) 6-BA with cotyledons as explants was 66%; (3) the optimum medium of shoot growth was MS medium plus 1.0 mg L(-1) 6-BA, 0.5 mg L(-1) NAA and 0.4 mg L(-1) GA; (4) the rooting rate was 60% on optimum rooting medium with 0.2 mg L(-1) NAA; (5) the survival rate of plant transplanting was 70% after transferred to pots with garden soil. In conclusion, the efficient plant regeneration system developed here will be helpful for rapid micropropagation and further genetic improvement in S. guianensis Sw. cv. 'Reyan 2'.

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[1]Plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis. Zhou, Quan-Nan,Huang, Tian-Dai,Li, Wei-Guo,Sun, Ai-Hua,Dai, Xue-Mei,Li, Zhe,Jiang, Ze-Hai. 2010

[2]Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis. Tan, Deguan,Sun, Xuepiao,Zhang, Jiaming. 2011

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[4]Embryo induction and plant regeneration of Callerya speciosa (Fabaceae) through anther culture. Huang, Bilan,Xu, Li,Li, Kelie,Fu, Yunlu,Li, Zhiying.

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