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Studies of the genetic diversity of seven sweetsop (Annona squamosa L.) cultivars by amplified fragment length polymorphism analysis

文献类型: 外文期刊

作者: Zhao Zhichang 1 ; Hu Guibing 1 ; Ouyang Ruo 1 ; Liu Yunchun 1 ; Chen Yeyuan 2 ; Luo Shirong 2 ;

作者机构: 1.S China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China

2.CATAS, Trop Germplasm Resources Res Inst, Danzhou 571737, Hainan, Peoples R China

关键词: Sweetsop;DNA extraction;amplified fragment length polymorphism (AFLP);genetic diversity

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2011 年 10 卷 35 期

页码:

收录情况: SCI

摘要: Seven sweetsop germplasm resources were studied, and amplified fragment length polymorphism (AFLP) analysis system was established by detecting the results of several reactions, such as DNA extraction, enzyme restriction, ligation, preamplification and selective amplification in the reaction system of AFLP. The seven germplasm resources of sweetsop were studied by using AFLP technique, and UPGMA clustering results showed genetic distance of 0.572 similar to 0.818. Small genetic distance was 0.572 between 'Yuanhua' sweetsop and other sweetsop cultivars; high genetic distance was 0.818 between 'AP' and 'Fenglishijia' sweetsop. These results suggested that there was relative family relationship between them. This aims to study the family relationship in seven sweetsop germplasms resources, in order to select parents in sweetsop breeding and grafted rootstock.

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