文献类型： 外文期刊

作者： Zhao Zhichang ¹ ; Hu Guibing ¹ ; Ou Yangruo ¹ ; Liu Yunchun ¹ ; Yi Yang ² ; Chen Yeyuan ³ ;

作者机构： 1.S China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China

2.Sichuan Univ, Sch Life Sci, Minist Educ, Key Lab Bioresources & Ecoenvironm, Chengdu 610064, Sichuan, Peoples R China

3.CATAS, Trop Germplasm Resource Res Inst, Danzhou 571737, Hainan, Peoples R China

关键词： Earlier identification;seedless wampee;bulked segregant analysis;randomly amplified polymorphic DNA

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2010 年 9 卷 50 期

页码：

收录情况： SCI

摘要： Wampee as an important fruit is widely grown in South China and conventional breeding methods for the production of new seedless wampee cultivars in recent years were operated. Hybridization was conducted using the seed as female parents and the seedless as male parents. However, the seeds could be gotten from cross combination. An efficient operation was that, non-woven fabrics bag could increase burliness rate in hybridization. In order to earlier identify seedless seedlings in hybrid progeny, the objective of this work is to identify molecular markers linked to seedless trait through bulked segregant analysis. Two bulks, seedless and seed, were prepared by pooling DNA of individual identified plants and used in polymerase chain reaction (PCR) analysis. Out of the 100 primers tested, only S06 primer could get a specific 930 bp restriction random amplified polymorphic DNA (RAPD amplicon to seedless bulk. As a result, a hybrid was tested with this primer in two cross combinations.