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Improved Production of Raw Starch Degrading Enzyme by Aspergillus oryzae F-30 Using Methyl Glucoside Sesqui-Stearate

文献类型： 外文期刊

作者： Sun, Hai-Yan 1 ; Wang, Lu 2 ; Liu, Jian-Wen 3 ; Peng, Ming 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, State Key Lab Trop Corp Biotechnol, Inst Trop Biosci & Biotechnol, Danzhou 571101, Hainan, Peoples R China

2.Wuxi Sci Res & Designing Inst State Adm Grain Res, Wuxi 214036, Jiangsu, Peoples R China

3.AsiaPac Biotechnol Co Ltd, Res Ctr Enzymes, Dongguan 523808, Guangdong Prov, Peoples R China

关键词： Aspergillus oryzae;Methyl glucoside sesqui-stearate;Raw starch degrading enzyme;Transcriptional level;Translational level

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2009 年 159 卷 1 期

页码：

收录情况： SCI

摘要： The effect of methyl glucoside sesqui-stearate (MGS) on the production of raw starch degrading enzyme (RSDE) by Aspergillus oryzae F-30 was studied in this paper. The activity of RSDE formed by Aspergillus oryzae F-30 was enhanced dramatically by the addition of MGS to the medium. As a result, with the addition of 1.5 g MGS in 1 L basal medium, RSDE activity and productivity were 107 U mL(-1) and 1.49 U mL(-1) h(-1), 4.3-fold and 7.1-fold greater than the values obtained in the basal medium, respectively. The effect of MGS on the synthesis of RSDE by Aspergillus oryzae F-30 was also studied on a molecular level. It was observed that the regulation of RSDE synthesis in Aspergillus oryzae F-30 occurs at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of MGS at transcriptional level.

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