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ANALYSIS OF THE GENETIC RELATIONSHIP BETWEEN PIPER METHYSTICUM AND PEPPER BY AFLP

文献类型： 外文期刊

作者： Shi, Jiang 1 ; Xin, Li 2 ; Yang, Yan 3 ; Zheng, Xueqin 1 ; Kong, Xiangsheng 2 ; Li, Zhiyong 2 ; Wang, Xiaoling 2 ; Gao, S 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, State Key Biotechnol Lab Trop Crops, Haikou 571101, Hainan, Peoples R China

2.Henan Univ Sci & Technol, Coll Agr, Luyong 471003, Henan, Peoples R China

3.Beijing Dingguo Biotechnol Co Ltd, Beijing 100088, Peoples R China

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）

ISSN： 0556-3321

年卷期： 2009 年 41 卷 3 期

页码：

收录情况： SCI

摘要： The aim of the research was to discuss the genetic relationships among Piper methysticum, Pepper and it wild relatives. With 28 germplasms including 6 materials of P. methysticum, 21 materials of cultivated and wild pepper, 1 material of Peperomia pellucida belonged to different genus as tested materials, DNA was extracted from leaves and primers with good band-type and high polymorphism and resolving power were screened from 64 pairs of primers for AFLP amplification and the clustering analysis was conducted with MVSP3.13f software. 191 bands were amplified by 4 pairs of primers, 189 of which had polymorphism being 98.6%. 28 germplasms were clustered into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pipper L., Piperaceae and were distantly related to the others. The research provided the basis for selecting rootstocks, molecular identification and the fingerprint construction of P. methysticum.

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