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Cloning and characterization of a Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus aidingensis AD-6(T)

文献类型： 外文期刊

作者： Zou, Ya Jie 1 ; Yang, Li Fu 1 ; Wang, Lei 1 ; Yang, Su Sheng 1 ;

作者机构： 1.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Beijing 100094, Peoples R China

2.Minist Agr, Key Lab Agromicrobial Resource & Applicat, Beijing 100094, Peoples R China

3.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou Hainan 571737, Peoples R China

4.Minist Agr, Key Lab Physiol Trop Crops, Danzhou Hainan 571737, Peoples R China

关键词： Halobacillus aidingensis;Na+/H+ antiporter;nhaH;inverse PCR

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN： 1225-8873

年卷期： 2008 年 46 卷 4 期

页码：

收录情况： SCI

摘要： A gene encoding a Na+/H+ antiporter was obtained from the genome of Halobacillus aidingensis AD-6(T), which was sequenced and designated as nhaH. The deduced amino acid sequence of the gene was 91% identical to the NhaH of H. dabanensis, and shared 54% identity with the NhaG of Bacillus subtilis. The cloned gene enable the Escherichia coli KNabc cell, which lack all of the major Na+/H+ antiporters, to grow in medium containing 0.2 M NaCl or 10 mM LiCl. The nhaH gene was predicted to encode a 43.5 kDa protein (403 amino acid residues) with 11 putative transmembrane regions. Everted membrane vesicles prepared from E. coli KNabc cells carrying NhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with the highest activity at pH 8.0, and no K+/H+ antiporter activity was detected. The deletion of hydrophilic C-terminal amino acid residues showed that the short C-terminal tail was vital for Na+/H+ antiporter activity.

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[1]A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization. Yang, LF,Jiang, JQ,Zhao, BS,Zhang, B,Feng, DQ,Lu, WD,Wang, L,Yang, SS.

[2]A primary sodium pump gene of the moderate halophile Halobacillus dabanensis exhibits secondary antiporter properties. Yang, LF,Jiang, JQ,Zhang, B,Zhao, BS,Wang, L,Yang, SS.

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