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Identification of differentially expressed proteins in poplar leaves induced by Marssonina brunnea f. sp Multigermtubi

文献类型： 外文期刊

作者： Yuan, Kun 1 ; Zhang, Bo 1 ; Zhang, Yanmei 1 ; Cheng, Qiang 1 ; Wang, Mingxiu 1 ; Huang, Minren 1 ;

作者机构： 1.Nanjing Forestry Univ, Key Lab Forest Genet & Gene Engn, Nanjing 210037, Peoples R China

2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Peoples R China

3.Chinese Acad Trop Agr Sci, S Subtrop Crops Res Inst, Zhanjiang 524091, Peoples R China

关键词： black spot disease;defense response;two-dimensional gel electrophoresis (2-DE);matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS);poplar

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2008 年 35 卷 1 期

页码：

收录情况： SCI

摘要： Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar (M. brunnea) interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone "NL895" (P. euramericana CL"NL895"), which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. multigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE. About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and unclassified proteins.

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