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Phylogenetic diversity of bacteria and Archaea associated with the marine sponge Pachychalina sp.

文献类型： 外文期刊

作者： Cheng, Na 1 ; Fang, Zaiguang 1 ; Huang, Huiqin 1 ; Fang, Zhe 1 ; Wu, Xiaopeng 1 ; Bao, Shixiang 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

关键词： marine sponges;diversity;bacteria;Archaea;ARDRA;Pachychalina sp.

期刊名称：POLISH JOURNAL OF ECOLOGY （ 影响因子：0.431； 五年影响因子：0.803 ）

ISSN： 1505-2249

年卷期： 2008 年 56 卷 3 期

页码：

收录情况： SCI

摘要： Molecular techniques were employed to document the microbial diversity associated with the marine sponge Pachychalina sp. from South China Sea in March 2003. Using the total microbial DNA as template, bacterial and archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned, sequenced and secondarily amplified by PCR. Then the secondarily amplified products were purified to be further characterized by amplified rDNA restriction analysis (ARDRA). According to the enzyme restriction mapping, the apparent difference among them were disclosed. 22 bacterial cloned partial sequences were acquired and most of them were related to proteobacterium. Also, 7 archaeal cloned partial sequences were acquired and a phylogenetic tree was built up. Result shows the prolific bacterial and archaeal diversity of marine sponge Pachychalina sp..

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