文献类型： 外文期刊

作者： Wei, Lijuan ¹ ; An, Zeshan ³ ; Mason, Annaliese S. ⁴ ; Xiao, Meili ¹ ; Guo, Ying ⁵ ; Yin, Jiaming ¹ ; Li, Jiana ¹ ; Fu, D ¹ ;

作者机构： 1.Southwest Univ, Chongqing Engn Res Ctr Rapeseed, Coll Agron & Biotechnol, Chongqing 400716, Peoples R China

2.Jiangxi Agr Univ, Agron Coll, Minist Educ, Key Lab Crop Physiol Ecol & Genet Breeding, Nanchang 330045, Peoples R China

3.Gansu Acad Agr Sci, Crops Res Inst, Lanzhou 730070, Gansu, Peoples R China

4.Univ Queensland, Ctr Integrat Legume Res, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia

5.Gansu Acad Agr Sci, Wheat Res Inst, Lanzhou 730070, Gansu, Peoples R China

关键词： Brassica napus;tRNA gene;Allopolyploidization;Sequence variation

期刊名称：JOURNAL OF MOLECULAR EVOLUTION （ 影响因子：2.395； 五年影响因子：2.282 ）

ISSN： 0022-2844

年卷期： 2014 年 78 卷 1 期

页码：

收录情况： SCI

摘要： Allopolyploidization, where two species come together to form a new species, plays a major role in speciation and genome evolution. Transfer RNAs (abbreviated tRNA) are typically 73-94 nucleotides in length, and are indispensable in protein synthesis, transferring amino acids to the cell protein synthesis machinery (ribosome). To date, the regularity and function of tRNA gene sequence variation during the process of allopolyploidization have not been well understood. In this study, the inter-tRNA gene corresponding to tRNA amplification polymorphism method was used to detect changes in tRNA gene sequences in the progeny of interspecific hybrids between Brassica rapa and B. oleracea, mimicking the original B. napus (canola) species formation event. Cluster analysis showed that tRNA gene variation during allopolyploidization did not appear to have a genotypic basis. Significant variation occurred in the early generations of synthetic B. napus (F-1 and F-2 generations), but fewer alterations were observed in the later generation (F-3). The variation-prone tRNA genes tended to be located in AT-rich regions. BlastN analysis of novel tRNA gene variants against a Brassica genome sequence database showed that the variation of these tRNA-gene-associated sequences in allopolyploidization might result in variation of gene structure and function, e.g., metabolic process and transport.