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Isolation, chromosomal location, and expression analysis of putative powdery mildew resistance genes in wheat (Triticum aestivum L.)

文献类型： 外文期刊

作者： Wan, Ping 1 ; Ling, Lijun 2 ; Cao, Shuanghe 2 ; Wang, Xianping 2 ; Zhang, Wenjun 3 ; Ling, Hongqing; Zhu, Lihuang;

作者机构： 1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

2.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; Gansu Acad Agr Sci, Lanzhou 730070, Peoples R China; China Agr Univ, Dept Plant Sci & Technol, Beijing 102206, Peoples R China

3.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; Gansu Acad Agr Sci, Lanzhou 730070, Peoples R Chin

关键词： Triticum aestivum;powdery mildew resistance;Mla-ortholog;gene cloning;chromosome location;expression

期刊名称：EUPHYTICA （ 2020影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2007 年 155 卷 1-2 期

页码：

收录情况： SCI

摘要： Special and degenerate primers are designed according to the conservative sequence of barley powdery mildew resistance genes Mla1, Mla6, and Mla13. Two wheat Mla-like orthologs, TaMla-2 and TaMla-3 are cloned and sequenced from the cDNA of wheat resistant-powdery mildew line TAM104R by RT-PCR method. TaMla-2 and TaMla-3 encode distinct but highly related coiled-coil nucleotide-binding site leucine-rich repeat type (NBS-LRR) resistant disease proteins and both reveal about 74 and 81% identity with amino acid sequence of Mla1, respectively. They are multiple copies in wheat genomes, one copy of them is mapped on wheat chromosome 1AL and two on 1BL using Chinese Spring nulli-tetra-somic lines and ditelosomic lines of 1A, 1B and 1D in southern analysis. This result suggests that may be the two Mla-like genes originated from the two diploid ancestral genomes, respectively. The expression pattern analysis of semi-quantitative PCR shows the TaMla genes are mainly expressed in leaf and sheath, and expression level is enhanced in organs infected by Erysiphe graminis, suggesting that TaMla-2 and TaMla-3 are powdery mildew resistance related-genes in wheat.

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