您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of Different PCR-Based Assays and LAMP Method for Rapid Detection of Phytophthora infestans by Targeting the Ypt1 Gene

文献类型： 外文期刊

作者： Khan, Mehran 1 ; Li, Benjin 2 ; Jiang, Yue 1 ; Weng, Qiyong 1 ; Chen, Qinghe 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Plant Protect, Fujian Key Lab Monitoring & Integrated Management, Fuzhou, Fujian, Peoples R China

关键词： LAMP;Phytophthora infestans;real-time PCR;sensitivity;specificity;Ypt1

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases affecting potato and tomato worldwide. Early diagnosis of the P. infestans pathogen causing late blight should be the top priority for addressing disease epidemics and management. In this study, we performed a loop-mediated isothermal amplification (LAMP) assay, conventional polymerase chain reaction (PCR), nested PCR, and real-time PCR to verify and compare the sensitivity and specificity of the reaction based on the Ypt1 (Ras-related protein) gene of P. infestans. In comparison with the PCR-based assays, the LAMP technique led to higher specificity and sensitivity, using uncomplicated equipment with an equivalent time frame. All 43 P. infestans isolates, yielded positive detection results using LAMP assay showing no cross reaction with other Phytophthora spp., oomycetes or fungal pathogens. The LAMP assay yielded the lowest detectable DNA concentration (1.28 x 10(-4) ng mu L-1), being 10 times more sensitive than nested PCR (1.28 x 10(-3) ng mu L-1), 100 times more sensitive than real-time PCR (1.28 x 10(-2) ng mu L-1) and 10(3) times more sensitive than the conventional PCR assay (1.28 x 10(-1) ng mu L-1). In the field experiment, the LAMP assay outperformed the other tests by amplifying only diseased tissues (leaf and stem), and showing no positive reaction in healthy tissues. Overall, the LAMP assay developed in this study provides a specific, sensitive, simple, and effective visual method for detection of the P. infestans pathogen, and is therefore suitable for application in early prediction of the disease to reduce the risk of epidemics.

  • 相关文献

[1]Specific and Sensitive Detection of Phytophthora nicotianae by Nested PCR and Loop-mediated Isothermal Amplification Assays. Li, Benjin,Liu, Peiqing,Xie, Shiyong,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe,Chen, Qinghe.

[2]Development and Evaluation of a Reverse Transcription-Loop-Mediated Isothermal Amplification Method for Rapid Detection of Novel Duck Reovirus. Li, Zhao-Long,Chen, Shi-Long,Chen, Shao-Ying,Lin, Feng-Qian,Cheng, Xiao-Xia,Wang, Shao,Zhu, Xiao-Li,Jiang, Bin,Li, Zhao-Long,Chen, Shi-Long,Chen, Shao-Ying,Lin, Feng-Qian,Cheng, Xiao-Xia,Wang, Shao.

[3]南洋臀纹粉蚧生物学特性及LAMP检测. 黄鹏,张杰,姚锦爱,蓝炎阳,余德亿. 2023

[4]鸭腺病毒3型LAMP快速检测方法的建立. 陈翠腾,黄瑜,陈珍,朱春华,傅光华,程龙飞,刘荣昌,刘斌琼,万春和. 2022

[5]Phenotypic and Genotypic Characterization of Phytophthora infestans Isolates from China. Li, Benjin,Chen, Qinghe,Lv, Xin,Lan, Chengzhong,Zhao, Jian,Qiu, Rongzhou,Weng, Qiyong.

[6]Loop-mediated isothermal amplification assay for sensitive and rapid detection of Phytophthora capsici. Dong, Zhongmei,Liu, Peiqing,Li, Benjin,Chen, Guoliang,Weng, Qiyong,Chen, Qinghe,Dong, Zhongmei,Chen, Guoliang,Weng, Qiyong,Chen, Qinghe.

[7]Development and evaluation of ITS- and aflP-based LAMP assays for rapid detection of Aspergillus flavus in food samples. Liu, Peiqing,Li, Benjin,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe.

[8]Microbial responses in a cold waterlogged paddy field to different distances from a drainage ditch. Fang, Yu,Wang, Fei,Chen, Jichen,Lin, Cheng,Lin, Xinjian. 2017

[9]The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus. Wan, Chunhe,Huang, Yu,Cheng, Longfei,Fu, Guanghua,Shi, Shao-hua,Chen, Hongmei,Peng, Chunxiang,Lin, Fang,Lin, Jiansheng. 2011

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台