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Screening of Vip3Aa60 and Vip3Ad5 and characterization of their binding to Spodoptera exigua midguts

文献类型： 外文期刊

作者： Pan, Zhi-Zhen 1 ; Xu, Lian 2 ; Zhang, Jing 2 ; Liu, Bo 3 ; Chen, Qing-Xi 2 ; Gao, Huan-Juan 2 ; Chen, Zheng 3 ; Zhu, Yu-J 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Key Lab Biopesticide & Chem Biol, State Key Lab Ecol Control Fujian Taiwan Crop Pes, Minist Educ, Fuzhou 350002, Fujian, Peoples R China

2.Xiamen Univ, Sch Life Sci, Minist Educ Coastal & Wetland Ecosyst, Key Lab, Xiamen 361005, Peoples R China

3.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Fujian, Peoples R China

关键词： Vip3Aa60;Vip3Ad5;Insecticidal activity;Spodoptera exigua;Bingding properties

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2017 年 61 卷

页码：

收录情况： SCI

摘要： Vip3A proteins, which shared no sequence or structural homology with the crystal proteins, were considered as a new generation of Bacillus thuringiensis toxins. In this study, we screened two novel vip3A genes from a number of B. thuringiensis strains, which were classified as vip3Aa60 and vip3Ad5 respectively. The full length of Vip3Aa60 and Vip3Ad5 showed 85% identity and their C-terminal domains showed 79.05% homology. The activity of both toxins was estimated against important lepidopteran pest (Spodoptera exigua). Vip3Aa60 revealed high toxicity against S.exigua as the LC50 value was 108.92 ng cm (-2) and Vip3Ad5 displayed low toxicity against S.exigua (LC50 > 4000 ng cm (-2)). In their binding assays, both Vip3Aa60 and Vip3Ad5 could bind to the Brush Border Membrane Vesicles (BBMV) of S. exigua and the affinity of binding to BBMV was significantly higher for Vip3Aa60 than Vip3Ad5. These results may provide useful information for insecticidal mechanism research of Vip3A against S. exigua.

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[1]Insecticidal activity and antifeedant effect of a new type biocide GCSC-BtA against Plutella xylostella L. (Lep., Plutellidae). Sengonca, C,Liu, B,Zhu, YJ. 2006

[2]Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.. Pan, Zhizhen,Zhu, Yujing,Shi, Huai,Chen, Zheng,Chen, Meichun,Liu, Bo,Xu, Lian,Chen, Qingxi.

[3]Proteolytic Activation of Bacillus thuringiensis Cry2Ab through a Belt-and-Braces Approach. Xu, Lian,Zhang, Jing,Chen, Qing-Xi,Pan, Zhi-Zhen,Liu, Bo,Zhu, Yu-Jing.

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