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Interplay between Alternative Splicing and Alternative Polyadenylation Defines the Expression Outcome of the Plant Unique OXIDATIVE TOLERANT-6 Gene

文献类型： 外文期刊

作者： Li, Qingshun Q. 1 ; Liu, Zhaoyang 4 ; Lu, Wenjia 2 ; Liu, Man 4 ;

作者机构： 1.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350003, Fujian, Peoples R China

2.Xiamen Univ, Coll Environm & Ecol, Minist Educ Coastal & Wetland Ecosyst, Key Lab, Xiamen 361102, Fujian, Peoples R China

3.Western Univ Hlth Sci, Grad Coll Biomed Sci, Pomona, CA 91766 USA

4.Miami Univ, Dept Biol, Oxford, OH 45056 USA

5.Xiamen Univ, Coll Environm & Ecol, Xiamen 361102, Fujian, Peoples R China

6.Univ Texas Austin, Dell Med Sch, Dept Pediat, Austin, TX 78723 USA

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Pre-mRNA alternative splicing and alternative polyadenylation have been implicated to play important roles during eukaryotic gene expression. However, much remains unknown regarding the regulatory mechanisms and the interactions of these two processes in plants. Here we focus on an Arabidopsis gene OXT6 (Oxidative Tolerant-6) that has been demonstrated to encode two proteins through alternative splicing and alternative polyadenylation. Specifically, alternative polyadenylation at Intron-2 of OXT6 produces a transcript coding for AtCPSF30, an Arabidopsis ortholog of 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. On the other hand, alternative splicing of Intron-2 generates a longer transcript encoding a protein named AtC30Y, a polypeptide including most part of AtCPSF30 and a YT521B domain. To investigate the expression outcome of OXT6 in plants, a set of mutations were constructed to alter the splicing and polyadenylation patterns of OXT6. Analysis of transgenic plants bearing these mutations by quantitative RT-PCR revealed a competition relationship between these two processes. Moreover, when both splice sites and poly(A) signals were mutated, polyadenylation became the preferred mode of OXT6 processing. These results demonstrate the interplay between alternative splicing and alternative polyadenylation, and it is their concerted actions that define a gene's expression outcome.

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