您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The regulatory role of Pcf11-similar-4 (PCFS4) in Arabidopsis development by genome-wide physical interactions with target loci

文献类型： 外文期刊

作者： Xing, Denghui 1 ; Wang, Yajun 1 ; Xu, Ruqiang 1 ; Ye, Xinfu 2 ; Yang, Dewei 2 ; Li, Qingshun Q. 1 ;

作者机构： 1.Miami Univ, Dept Bot, Oxford, OH 45056 USA

2.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou, Fujian, Peoples R China

3.Xiamen Univ, Key Lab, Minist Educ Coastal & Wetland Ecosyst, Xiamen 361102, Fujian, Peoples R China

4.Xiamen Univ, Coll Environm & Ecol, Xiamen 361102, Fujian, Peoples R China

关键词： Transcription;Polyadenylation factor;RNA processing;Alternative processing;ChIP-seq

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2013 年 14 卷

页码：

收录情况： SCI

摘要： Background: The yeast and human Pcf11 functions in both constitutive and regulated transcription and pre-mRNA processing. The constitutive roles of PCF11 are largely mediated by its direct interaction with RNA Polymerase II C-terminal domain and a polyadenylation factor, Clp1. However, little is known about the mechanism of the regulatory roles of Pcf11. Though similar to Pcf11 in multiple aspects, Arabidopsis Pcf11-similar-4 protein (PCFS4) plays only a regulatory role in Arabidopsis gene expression. Towards understanding how PCFS4 regulates the expression of its direct target genes in a genome level, ChIP-Seq approach was employed in this study to identify PCFS4 enrichment sites (ES) and the ES-linked genes within the Arabidopsis genome. Results: A total of 892 PCFS4 ES sites linked to 839 genes were identified. Distribution analysis of the ES sites along the gene bodies suggested that PCFS4 is preferentially located on the coding sequences of the genes, consistent with its regulatory role in transcription and pre-mRNA processing. Gene ontology (GO) analysis revealed that the ES-linked genes were specifically enriched in a few GO terms, including those categories of known PCFS4 functions in Arabidopsis development. More interestingly, GO analysis suggested novel roles of PCFS4. An example is its role in circadian rhythm, which was experimentally verified herein. ES site sequences analysis identified some over-represented sequence motifs shared by subsets of ES sites. The motifs may explain the specificity of PCFS4 on its target genes and the PCFS4's functions in multiple aspects of Arabidopsis development and behavior. Conclusions: Arabidopsis PCFS4 has been shown to specifically target on, and physically interact with, the subsets of genes. Its targeting specificity is likely mediated by cis-elements shared by the genes of each subset. The potential regulation on both transcription and mRNA processing levels of each subset of the genes may explain the functions of PCFS4 in multiple aspects of Arabidopsis development and behavior.

  • 相关文献

[1]Genome-wide determination of poly(A) sites in Medicago truncatula: evolutionary conservation of alternative poly(A) site choice. Wu, Xiaohui,Gaffney, Bobby,Hunt, Arthur G.,Li, Qingshun Q.,Li, Qingshun Q.,Li, Qingshun Q.. 2014

[2]Plant polyadenylation factors: conservation and variety in the polyadenylation complex in plants. Hunt, Arthur G.,Xing, Denghui,Li, Qingshun Q.,Li, Qingshun Q.,Li, Qingshun Q.. 2012

[3]Genome-wide characterization of intergenic polyadenylation sites redefines gene spaces in Arabidopsis thaliana. Wu, Xiaohui,Zeng, Yong,Guan, Jinting,Ji, Guoli,Huang, Rongting,Li, Qingshun Q.,Li, Qingshun Q.,Li, Qingshun Q.,Ji, Guoli. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台