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Identification of differentially expressed genes implicated in peel color (red and green) of Dimocarpus confinis

文献类型： 外文期刊

作者： Jiang, Fan 1 ; Chen, Xiu-ping 1 ; Hu, Wen-shun 1 ; Zheng, Shao-quan 1 ;

作者机构： 1.Fujian Fruit Breeding Engn Technol Res Ctr Longan, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Fujian, Peoples R China

关键词： Dimocarpus confinis;Peel color;Anthocyanins (ACs);Sequence analysis;Differentially expressed genes (DEGs)

期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）

ISSN： 2193-1801

年卷期： 2016 年 5 卷

页码：

收录情况： SCI

摘要： Nowadays, there are few reports about regulatory genes implicated in peel color of longan. The basic genetic research of longan has been in stagnation for a long time as a lack of transcriptomic and genetic information. To predict candidate genes associated with peel color, Gene Functional Annotation and Coding Sequence prediction were used to perform functional annotation for our assembled unigenes and investigate differentially expressed genes (DEGs) of fruitlet peels from Longli (Dimocarpus confinis). Finally, a total of 24,044 (44.19 %) unigenes were annotated at least in one database after BLAST search to NCBI non-redundant protein sequence, NCBI non-redundant nucleotide sequences, Kyoto Encyclopedia of Genes and Genomes (KEGG) Ortholog, manually annotated and reviewed protein sequence database (Swiss-Prot), Protein family, Gene Ontology, euKaryotic Ortholog Groups databases. After searching against the KEGG-GENE protein database, a result of 6228 (11.45 %) unigenes were assigned to 245 KEGG pathways. Via comparing the distributions of expression value of all corresponding unigenes from red peel and green peel fruit, it could be intuitively concluded that high similarity was existed in the two distributions; however, on the whole, between two distributions of log RPKM expression value, some differences indicated that expression level in green-peel fruit group is slightly higher than values in red-peel fruit group. Finally, a total of 1349 unigenes were identified as DEGs after blasting the DEGs to public sequence databases, and 32 peel-color-related genes were identified in longan. Our results suggest that a number of unigenes involved in longan metabolic process, including anthocyanin biosynthesis. In addition, DRF, F3H, ANS, CYP75A1 and C1 may be the key ones. The study on key genes related to peel color will be contributed to revealing the molecular mechanisms of regulating peel color in woody plants.

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