Characterization of a myrosinase cDNA from Brassica parachinensis and its defense role against Plutella xylostella after suppression
文献类型： 外文期刊
作者： Yang, Guang 1 ; Wang, Ding-Feng 1 ; Dong, Zhi-Quan 1 ; Wang, Qian-Liang 1 ; Wang-Pruski, Gefu 4 ; You, Min-Sheng 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, Fuzhou 350002, Peoples R China
2.Fujian Acad Agr Sci, Tea Res Inst, Fuan, Fujian, Peoples R China
3.Inst Prevent & Control Profess Dis, Fuzhou, Peoples R China
4.Nova Scotia Agr Coll, Dept Plant & Anim Sci, Truro, NS B2N 5E3, Canada
关键词： Brassica parachinensis;myrosinase;Plutella xylostella;RNA interference
期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）
ISSN： 1672-9609
年卷期： 2012 年 19 卷 4 期
页码：
收录情况： SCI
摘要： In Brassicaceae, myrosinase catalyzes the hydrolysis of glucosinolate and plays an important role in anti-herbivore defense. We have cloned and characterized the full-length complementary DNA of myrosinase gene from Brassica parachinensis that exhibits high sequence identity with myrosinase genes from other Brassica species. To investigate the role of this myrosinase in defense against the diamondback moth (Plutella xylostella), we constructed an RNA-interference (RNAi) cassette expressing a double-stranded RNA that targeted myrosinase and transfected it into B. parachinensis. Myrosinase was suppressed in the resulting transgenic plants. Diamondback moth larvae feeding on transgenic plants had lower larval and pupal weights, longer pupal duration, and lower fecundity than those feeding on non-transgenic plants, suggesting that the diamondback moth has adapted to the glucosinolate-myrosinase defensive system. Therefore, the suppression of myrosinase is a potential approach for controlling the diamondback moth.
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