您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses

文献类型： 外文期刊

作者： Fu, Guanghua 1 ; Liu, Mengda 1 ; Zeng, Wenshu 1 ; Pu, Juan 1 ; Bi, Yuhai 1 ; Ma, Guangpeng 3 ; Liu, Jinhua 1 ;

作者机构： 1.China Agr Univ, Key Lab Zoonosis, Coll Vet Med, Minist Agr, Beijing 100193, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

3.China Rural Technol Dev Ctr, Beijing 100045, Peoples R China

4.Shandong Anim Dis Control Ctr, Jinan 250022, Shandong, Peoples R China

关键词： Swine influenza virus;Classical swine H1N1;European avian-like H1N1;Human-like H3N2;Multiplex RT-PCR

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2010 年 41 卷 2 期

页码：

收录情况： SCI

摘要： Classical swine H1N1, emerging European avian-like H1N1 and human-like H3N2 lineages are co-circulating in the swine population in China. The reverse transcriptase polymerase chain reaction (RT-PCR) assay is an effective method for use in influenza surveillance. In this study, a multiplex RT-PCR method was developed for simultaneous identification of hemagglutinin (HA) genes derived from the three lineages of swine influenza viruses. Three primer sets were designed and aimed specifically at HA genes of these viral lineages. The specificity of the assay showed that the established methods could efficiently differentiate the HA genes of classical swine H1N1, European avian-like H1N1, and human-like H3N2 viruses while other viruses such as classical swine fever virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, and porcine circovirus type 2, could not be detected. The assay showed a sensitivity of 1 x 10(2.5) 50% egg infectious dose for each virus lineage. The comparison of the results with those obtained from the analysis of 300 swine tracheal swab samples by means of virus isolation showed a high level of agreement. This multiplex RT-PCR method provides a rapid and specific swine influenza diagnostic tool that also has the potential for investigating the epidemiology of different lineages of swine influenza virus prevalent currently in China.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台