文献类型： 外文期刊

作者： Wu, Hanxiang ¹ ; Marhadour, Sophie ² ; Lei, Zhi-Wei; Dugaro, Emilie ² ; Gaillard, Cecile ¹ ; Porcheron, Benoit ¹ ;

作者机构： 1.Univ Poitiers, CNRS, Equipe SEVE, Lab EBI,UMR 7267,TSA 51106, 3 Rue Jacques Ft, F-86073 Poitiers 9, France

2.Univ Poitiers, CNRS, IC2MP Inst Chim Milieux & Mat Poitiers, UMR 7285,TSA 51106, 4 Rue Michel Brunet, F-86073 Poitiers 9, France

3.Univ Poitiers, CNRS, IC2MP Inst Chim Milieux & Mat Poitiers, UMR 7285,TSA 51106, 4 Rue Michel

关键词： Apoplasmic loaders;Arabidopsis AtSUC2;D-glucose-fenpiclonil conjugate;PCMBS;phloem loading;Ricinus seedlings;specific inhibition of sucrose carriers;sucrose uptake from endosperm;Vicia faba

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2017 年 68 卷 20 期

页码：

收录情况： SCI

摘要： Until now, specific inhibitors of sucrose carriers were not available. This led us to study the properties of the recently synthesized D-glucose-fenpiclonil conjugate (D-GFC). This large amphiphilic glucoside exhibited an extremely low phloem systemicity in contrast to L-amino acid-fenpiclonil conjugates. Using Ricinus seedlings, the effect of D-GFC on 0.5 mM [C-14] sucrose (Suc), 3-O-[H-3] methylglucose, and [H-3] glutamine uptake by cotyledon tissues was compared with that of p-chloromercuribenzenesulfonic acid (PCMBS). D-GFC dramatically inhibited H+-Suc symport at the same concentrations as PCMBS (0.5 and 1 mM), but in contrast to the thiol reagent, it did not affect 3-O-methylglucose and glutamine transport, nor the acidification of the incubation medium by cotyledon tissues. Similarly, 0.5 mM D-GFC inhibited active Suc uptake by Vicia faba leaf tissues and by Saccharomyces cerevisiae cells transformed with AtSUC2, a gene involved in Suc phloem loading in Arabidopsis, by approximately 80%. The data indicated that D-GFC was a potent inhibitor of Suc uptake from the endosperm and of Suc phloem loading. It is the first chemical known to exhibit such specificity, at least in Ricinus, and this property