文献类型： 外文期刊
作者： Yang, Bing 1 ; Chen, Yi-Meng 2 ; Liu, Yong-Xiang 2 ; Liu, Zuo-Yi 2 ; Zhou, De-Qun 1 ;
作者机构： 1.Kunming Univ Sci & Technol, Fac Environm Sci & Engn, Kunming 650500, Peoples R China
2.Guizhou Acad Agr Sci, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China
3.Fuxin Meteorol Bur Mongolia Autonomous Cty, Fuxing 123100, Peoples R China
关键词： Shiraia Bambusicola;AFLP;System Optimizing;Polymorphic Primer
期刊名称：PROCEEDINGS OF THE 2015 INTERNATIONAL CONFERENCE ON MATERIAL SCIENCE AND APPLICATIONS (ICMSA 2015)
ISSN： 2352-541X
年卷期： 2015 年 3 卷
页码：
收录情况： SCI
摘要： DNA of Sharaia bambuiscola was extracted by the improved method of CTAB. The several key factors affecting the effect of DNA digestion and the PCR selective amplification such as the time of DNA digestion, the times of the per-amplified dilute products, the mount of the selective amplification primer, Taq concentration and dNTPs concentration were optimized and trialed with establishment of an optimized AFLP reaction system of S. bambusicola. The best time of digestion DNA with double endonucleases (EcoR I and Mse I) was 3h. The optimized selection amplification system was 25 mu l reaction mix containing 2.5 mu l the 20 times of the per-amplified dilute products, 2.5 mu L 10x buffer(with Mg2+), 10mmol/L primer each 1.5 mu L, 2.5U.mu L-1 Taq polymerase 0.7 mu L, 10mmol/L dNTP 0.5 mu L, and 15.8 mu L ddH(2)O. Stable and clean DNA finger print can be obtained and 3 pairs of AFLP primers with good genetic diversity were selected according to the optimized reaction system. The results will be an effective protocol for further studying the genetic stucture and differentiation, phylogenetic trees, host specificity and artificial cultivation of Shiraia bambusicola population.
- 相关文献
作者其他论文 更多>>
-
Phylogeny and taxonomy of two new species in Dictyosporiaceae (Pleosporales, Dothideomycetes) from Guizhou, China
作者：Feng, Yao;Yang, Mi-Lian;Feng, Yao;Liu, Zuo-Yi;Chen, Xiao-Fang;Zhang, Zhi-Yuan;Chen, Ya -Ya;Chen, Ya -Ya
关键词：2 new species; Gregarithecium; multi-locus; new taxa; Pseudocoleophoma; taxonomy
-
Freshwater fungi from karst landscapes in China and Thailand
作者：Yang, Jing;Liu, Ning-Guo;Li, Wen-Li;Yu, Xian-Dong;Liu, Jian-Kui;Yang, Jing;Hyde, Kevin D.;Bao, Dan-Feng;Liu, Ning-Guo;Shen, Hong-Wei;Yang, Jing;Liu, Zuo-Yi;Liu, Ling-Ling;Jones, E. B. Gareth;Jones, E. B. Gareth;Bao, Dan-Feng;Hyde, Kevin D.;Liu, Zuo-Yi;Liu, Ning-Guo
关键词：58 new taxa; Asexual morph; Multi-gene; Phylogeny; Sexual morph; Taxonomy
-
Ascomycetes from karst landscapes of Guizhou Province, China
作者：Zhang, Jin-Feng;Zhang, Jin-Feng;Chen, Ya-Ya;Liu, Zuo-Yi;Zhang, Jin-Feng;Hyde, Kevin D.;Liu, Jian-Kui;Hyde, Kevin D.;Liu, Zuo-Yi;Chen, Ya-Ya;Ran, Hai-Yan
关键词：49 new taxa; Dothideomycetes; Multi-gene; Phylogeny; Sordariomycetes; Taxonomy
-
Triplosphaeria guizhouensis sp. nov. (Tetraplosphaeriaceae, Pleosporales), a novel taxon from freshwater habitat in Guizhou Province, China
作者：Liu, Ling-Ling;Wei, Quan-Quan;Zhang, Meng;Gu, Xiao-Feng;Liu, Zuo-Yi;Gou, Jiu-Lan;Liu, Ling-Ling;Feng, Yao;Liu, Zuo-Yi;Feng, Yao
关键词：1 new taxon; molecular phylogeny; Tetraploa -like; anamorph; setose appendages
-
Dictyosporium duliujiangense sp. nov. (Dictyosporiaceae, Pleosporales) from freshwater habitat in Guizhou Province, China
作者：Liu, Ling-Ling;Gu, Xiao-Feng;Wei, Quan-Quan;Zhang, Meng;Gou, Jiu-Lan;Liu, Ling-Ling;Song, Lian-Chai;Liu, Zuo-Yi;Song, Lian-Chai
关键词：1 new taxon; asexual fungi; sporodochia; cheiroid conidia; molecular phylogeny
-
Curvicladiella paphiopedili sp. nov. (Hypocreales, Nectriaceae), a new species of orchid (Paphiopedilum sp.) from Guizhou, China
作者：Song, Lian-chai;Song, Lian-chai;Wang, Li-li;Huang, Lu;Liu, Ling-ling;Feng, Yao;Liu, Zuo-yi
关键词：Curvicladiella; morphology; phylogeny; Paphiopedilum; taxonomy
-
Fissuroma bambucicola sp. nov. (Aigialaceae, Pleosporales) from Bamboo in Guizhou, China
作者：Feng, Yao;Feng, Yao;Feng, Yao;Liu, Zuo-Yi;Zhang, Sheng-Nan;Chen, Ya-Ya
关键词：1 new taxon; Dothideomycetes; muti-gene; Phylogeny; Taxonomy
