文献类型： 外文期刊
作者： Zhu, G. S. 1 ; Yu, Z. N. 1 ; Gui, Y. 1 ; Liu, Z. Y. 1 ;
作者机构： 1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
2.Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Guizhou, Peoples R China
3.Guizhou Acad Agr Sci, Inst Biotechnol, Guiyang 550006, Guizhou, Peoples R China
关键词： Ceratorhiza;Epulorhiza;isolate;Monilioposis;mycorrhiza;orchid;peloton
期刊名称：FUNGAL DIVERSITY （ 影响因子：20.372； 五年影响因子：16.344 ）
ISSN： 1560-2745
年卷期： 2008 年 33 卷
页码：
收录情况： SCI
摘要： We describe a technique for isolating mycorrhizal fungi from roots of orchids. This technique involves selection and treatment of roots, preparation of pelotons, treatment of pelotons, Culture of pelotons so that fungal hyphae grow out and strain purification. The technique is considered better because 1) problems of fungal and bacterial contamination are resolved, 2) endophytic bacteria arc suppressed and also used to promote hyphal growth from the pelotons, 3) live and dead pelotons, and those from which fungi are culturable Or unculturable can easily be identified, providing increased isolation efficiency, 4) a single taxon can be isolated from a single peloton containing several mycorrhizal taxa, 5) slow-growing mycorrhizal taxa can easily be isolated. The implications and potential use of this technique in future studies is discussed.
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