文献类型： 外文期刊

作者： Wen, XP ¹ ; Deng, XX ² ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Guizhou Univ, Guizhou Key Lab Agr Biotechnol, Guiyang 550025, Peoples R China

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN： 1462-0316

年卷期： 2005 年 80 卷 1 期

页码：

收录情况： SCI

摘要： Chestnut rose (Rosa roxburghii Tratt) shows desirable prospects both in fruit exploitation and ornamental plant genetic improvement. A micropropagation system based on axillary bud proliferation was established and optimized for mature trees. Nodal segments, 30-45 d-old, gave the best regeneration after being incubated in half-strength MS medium supplemented with gibberellic acid (GA(3); 2.9 muM), 6-benzyladenine (BA; 2.2 muM), naphthalene acetic acid (NAA; 0.5 muM), and activated charcoal (AC; 300-400 mg 1(-1)). The best multiple-shoot response was obtained with 'Guinong No. 5' and 'white-flower' chestnut rose on a modified MS, medium supplemented with 2.9 muM GA(3), 2.2-4.4 muM BA, and 0.5 muM NAA. Shoots 4-5 cm in height, cultured for 20-25 d in 1/2 MS medium supplemented with 1.6 muM NAA, 2.5-3.5 muM paclobutrazol, and 300-400 mg 1(-1) AC showed the best rooting. No polymorphic marker was found among 669 RAPD markers generated with 124 arbitrary primers, in multiplications of 'Guinong No. 5'. To test further the genetic stability of in vitro shoots of 'Guinong No. 5' and 'white-flower' chestnut rose, a total of 1,307 and 1,339 AFLP markers, amplified with 54 selective primer combinations were scored. No aberrant marker profile was observed when in vitro shoots were multiplied through as many as 25 in vitro cycles. These results indicate that this in vitro culture system may provide a useful alternative for chestnut rose multiplication and conservation.