文献类型： 外文期刊

作者： Fu, Wenqin ¹ ; Chen, Daozong ¹ ; Pan, Qi ¹ ; Li, Fengfeng ¹ ; Zhao, Zhigang ² ; Ge, Xianhong ¹ ; Li, Zaiyun ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Ctr Oil Crop Improvement Wuhan, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China

2.Qinghai Univ, Qinghai Acad Agr & Forestry Sci, Xining, Peoples R China

关键词： Brassica napus;flower colour;anthocyanin;transcriptome;PAP2;Orychophragmus violaceus

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2018 年 16 卷 2 期

页码：

收录情况： SCI

摘要： Oilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower colour of oilseed rape. A previously established B. napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits slightly red petals. In this study, the transcriptomic analysis of M4, B. napus (H3), and O. violaceus with purple petals (OvP) and with white petals (OvW) revealed that most anthocyanin biosynthesis genes were up-regulated in both M4 and OvP. Read assembly and sequence alignment identified a homolog of AtPAP2 in M4, which produced the O. violaceus transcript (OvPAP2). The overexpression of OvPAP2 via the CaMV35S promoter in Arabidopsis thaliana led to different levels of anthocyanin accumulation in most organs, including the petals. However, the B. napus overexpression plants showed anthocyanin accumulation primarily in the anthers, but not the petals. However, when OvPAP2 was driven by the petal-specific promoter XY355, the transgenic B.napus plants produced red anthers and red petals. The results of metabolomic experiments showed that specific anthocyanins accumulated to high levels in the red petals. This study illustrates the feasibility of producing red-flowered oilseed rape, thereby enhancing its ornamental value, via the ectopic expression of the OvPAP2 gene. Moreover, the practical application of this study for insect pest management in the crop is discussed.