文献类型： 外文期刊

作者： Liu, H. D. ¹ ; Zhao, Z. G. ¹ ; Du, D. Z. ¹ ; Deng, C. R. ¹ ; Fu, G. ¹ ;

作者机构： 1.Qinghai Univ, Qinghai Acad Agr & Forestry Sci, Key Lab Spring Rapeseed Genet Improvement, Natl Key Lab Breeding Base Innovat & Utilizat Pla, Xining, Qinghai Provinc, Peoples R China

关键词： Allopolyploidization;Resynthesized Brassica napus;Genetic variation;Qinghai-Tibet Plateau

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 1 期

页码：

收录情况： SCI

摘要： This study aimed to reveal the genetic and epigenetic variations involved in a resynthesized Brassica napus (AACC) generated from a hybridization between a B. rapa (AA) landrace and B. alboglabra (CC). Amplified fragment length polymorphism (AFLP), methylation-sensitive amplified polymorphism, and the cDNA-AFLP technique were performed to detect changes between different generations at the genome, methylation, and transcription levels. We obtained 30 lines of resynthesized B. napus with a mean 1000-seed weight of over 7.50 g. All of the lines were self-compatible, probably because both parents were self-compatible. At the genome level, the S0 generation had the lowest frequency of variations (0.18%) and the S3 generation had the highest (6.07%). The main variation pattern was the elimination of amplified restriction fragments on the CC genome from the S0 to the S4 generations. At the methylation level, we found three loci that exhibited altered methylation patterns on the parental A genome; the variance rate was 1.35%. At the transcription level, we detected 43.77% reverse mutations and 37.56% deletion mutations that mainly occurred on the A and C genomes, respectively, in the S3 generation. Our results highlight the genetic variations that occur during the diploidization of resynthesized B. napus.