Quick detection of Carassius auratus herpesvirus (CaHV) by recombinase-aid amplification lateral flow dipstick (RAA-LFD) method
文献类型： 外文期刊
作者： Gui, Lang 1 ; Zhao, Yun 1 ; Xu, Dan 1 ; Li, Xinyu 1 ; Luo, Jianhua 2 ; Zhou, Wenzong 3 ; Li, Mingyou 1 ;
作者机构： 1.Shanghai Ocean Univ, Key Lab Integrated Rice Fish Farming, Minist Agr & Rural Affairs, Shanghai, Peoples R China
2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai, Peoples R China
3.Shanghai Acad Agr Sci, Ecoenvironm Protect Res Inst, Shanghai, Peoples R China
关键词： carassius auratus herpesvirus (CaHV); tumor necrosis factor receptor (TNFR); recombinase-aid amplification; lateral flow dipstick; point-of-care testing (POCT)
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：6.073； 五年影响因子：6.34 ）
ISSN： 2235-2988
年卷期： 2022 年 12 卷
页码：
收录情况： SCI
摘要： Crucian carp (Carassius auratus) is one of the major freshwater species and is also a common food fish in China. Recently, Carassius auratus herpesvirus (CaHV) could induce fatal viral disease with high mortality of crucian carp, which had caused huge economic losses. In this study, we described a rapid and simple recombinase-aid amplification (RAA) assay coupled with lateral flow dipstick (LFD), which could achieve sensitive diagnosis of tumor necrosis factor receptor (TNFR) of CaHV within 35 min at 40 degrees C. Our RAA-LFD method had a satisfactory detection limit of 100 gene copies per reaction, which was 100-fold more sensitive than traditional PCR. In addition, no cross-reaction was observed with other viral pathogens, including koi herpesvirus (KHV), cyprinid herpesvirus 2 (CyHV-2), infectious hematopoietic necrosis virus (IHNV), spring viremia of carp virus (SVCV) and grass carp reovirus (GCRV). Furthermore, the overall cost of the method was cut in half compared to previous studies. In conclusion, RAA-LFD assay is therefore, a promising alternative for point-of-care testing (POCT) of CaHV, which is feasible and of certain value in application of aquatic disease control.
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