文献类型： 外文期刊

作者： Xia, Yanling ¹ ; Qu, Haomiao ¹ ; Lu, Binshan ¹ ; Zhang, Qiang ¹ ; Li, Heping ¹ ;

作者机构： 1.Northeast Forestry Univ, Coll Wildlife Resources, Harbin 150040, Heilongjiang, Peoples R China

2.Heilongjiang Acad Agr Sci Postdoctoral Programme, Harbin 150086, Heilongjiang, Peoples R China

关键词： Sika Deer;Annexin A2;Cloning;Phylogenetic Analysis;Real Time Reverse Transcriptase Polymerase Chain Reaction

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN： 1011-2367

年卷期： 2018 年 31 卷 4 期

页码：

收录情况： SCI

摘要： Objective: Molecular cloning and bioinformatics analysis of annexin A2 (ANXA2) gene in sika deer antler tip were conducted. The role of ANXA2 gene in the growth and development of the antler were analyzed initially. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the ANXA2 gene from antler tip of sika deer (Cervus Nippon hortulorum) and the bioinformatics methods were applied to analyze the amino acid sequence of Anxa2 protein. The mRNA expression levels of the ANXA2 gene in different growth stages were examined by real time reverse transcriptase polymerase chain reaction (real time RT-PCR). Results: The nucleotide sequence analysis revealed an open reading frame of 1,020 bp encoding 339 amino acids long protein of calculated molecular weight 38.6 kDa and isoelectric point 6.09. Homologous sequence alignment and phylogenetic analysis indicated that the Anxa2 mature protein of sika deer had the closest genetic distance with Cervus elaphus and Bos mutus. Real time RT-PCR results showed that the gene had differential expression levels in different growth stages, and the expression level of the ANXA2 gene was the highest at metaphase (rapid growing period). Conclusion: ANXA2 gene may promote the cell proliferation, and the finding suggested Anxa2 as an important candidate for regulating the growth and development of deer antler.