Complete genome sequence of a novel lytic bacteriophage isolated from Ralstonia solanacearum
文献类型： 外文期刊
作者： Su, Jingfang 1 ; Sun, Hongwei 2 ; Liu, Junjie 1 ; Guo, Zhaokui 2 ; Fan, Guoquan 3 ; Gu, Gang 4 ; Wang, Guanghua 1 ;
作者机构： 1.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Heilongjiang, Peoples R China
2.China Natl Tobacco Corp, Heilongjiang Branch, Mudanjiang Tobacco Res Inst, Mudangjiang 157011, Peoples R China
3.Heilongjiang Acad Agr Sci, Virus Free Seedling, Harbin 150086, Heilongjiang, Peoples R China
4.Fujian Tobacco Monopoly Bur, Tobacco Agr Inst, Fuzhou 350003, Fujian, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2017 年 162 卷 12 期
页码：
收录情况： SCI
摘要： A lytic podophage RSPI1 was isolated from tobacco field soil collected in Fujian Province, South China using host bacterium Ralstonia solanacearum Tb15-14. Whole genome sequencing of this phage was performed using the high-throughput Ion Torrent PGM Sequencer. The complete genome of RSPI1 was 43,211 bp in length with a mean DNA G + C content of 61.5%. A total of 48 open reading frames were identified with lengths ranging from 132 bp to 5,061 bp, of which, 11, 12 and 25 were identified as functional, structural and unknown genes, respectively. A BLAST analysis revealed that this phage genome had a query cover of 78-79% and a highest identity of 84% with four podophages that infect Burkholderia pseudomallei. Two neighbor-joining phylogenetic trees were constructed using phage DNA polymerase I and tail fiber protein sequences and showed that this phage is closely related to Burkholderia phage Bp-AMP1, and also related to several phages that infect Ralstonia solanacearum. These findings indicate that RSPI1 is a novel phage that infects the notorious plant pathogen Ralstonia solanacearum.
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